有许多方法可用于确定您的杆状病毒库存的感染滴度。TCID50方法是一种简单的终点稀释方法，可确定产生细胞病变效应或杀死50％接种的昆虫细胞所需的杆状病毒病毒量。将连续稀释的杆状病毒原液添加到在96孔板中培养的Sf9细胞中，并在感染后3-5天，监测细胞的细胞死亡或细胞病变效应。然后可以通过该方法中所述的Reed-Muench方法计算滴度。
There are many methods that can be used to determine the infectious titer of your baculovirus stock. The TCID50 method is a simple end-point dilution method that determines the amount of baculovirus virus needed to produce a cytopathic effect or kill 50% of inoculated insect cells. Serial dilutions of baculovirus stock are added to Sf9 cells cultivated in 96-well plates and 3-5 days after infection, cells are monitored for cell death or cytopathic effect. The titer can then be calculated by the Reed-Muench method as described in this method.
