康复新因其用于治疗溃疡性疾病和黏膜炎而被广泛认可,主要是由于它的抗炎特性,促进细胞增殖,肉芽组织生长,和血管生成。然而,这些影响背后的确切机制仍然知之甚少。在这项研究中,我们采用高通量质谱鉴定了康复新中的11种化合物,包括尿嘧啶,次黄嘌呤,黄嘌呤,肌苷,谷氨酸,甘氨酸,丙氨酸,缬氨酸,异亮氨酸,亮氨酸,还有赖氨酸.值得注意的是,肌苷的解热和抗炎特性,这些化合物中的一种,没有得到很好的表征。为了解决这个差距,我们使用脂多糖(LPS)在体内诱发发热,并进行了各种实验,包括对内生介质的分析,炎症因子,定量聚合酶链反应(QPCR),西方印迹,苏木精和伊红(HE)染色。我们的发现表明肌苷显著降低LPS引起的发热,抑制炎症因子的表达,减轻炎症反应。这些结果表明肌苷可以作为炎症性疾病的潜在治疗靶标。
Kangfuxin has been widely recognized for its use in treating ulcerative conditions and mucositis, primarily due to its anti-inflammatory properties, which promote cell proliferation, granulation tissue growth, and angiogenesis. However, the exact mechanisms underlying these effects remain poorly understood. In this study, we employed high-throughput mass spectrometry to identify 11 compounds in Kangfuxin, including uracil, hypoxanthine, xanthine,
inosine, glutamic acid, glycine, alanine, valine, isoleucine, leucine, and lysine. Notably, the antipyretic and anti-inflammatory properties of
inosine, one of these compounds, have not been well characterized. To address this gap, we induced fever in vivo using lipopolysaccharide (LPS) and conducted various experiments, including the analysis of endogenous mediators, inflammatory factors, quantitative polymerase chain reaction (QPCR), Western blotting, and hematoxylin and eosin (HE) staining. Our findings indicate that
inosine significantly reduces LPS-induced fever, inhibits the expression of inflammatory factors, and alleviates the inflammatory response. These results suggest that
inosine may serve as a potential therapeutic target for inflammatory diseases.