背景:我们进行了混合作图和精细作图分析,以确定影响认知能力的起源基因位点。
方法:我们在7140个不同的西班牙裔和拉丁裔成年人(平均年龄55岁)中估计了整个基因组中局部祖先间隔与5项神经认知测量的关联。我们优先考虑相关基因座中的遗传变异,并测试它们在四个独立队列中的复制。
结果:我们确定了五种神经认知测量的9个局部祖先相关区域。在所有基因座观察到的与认知功能的关联都有很强的生物学支持,并且在4q12、9p22.1和13q12.13处存在独立复制的统计证据。
结论:我们的研究发现了多个新基因位点,这些基因与认知功能和痴呆有关。并发现了与祖先相关的遗传变异。它增加了我们对西班牙裔和拉丁裔成年人认知功能的遗传结构的理解,并展示了混合物图谱的力量,以发现影响认知功能的独特单倍型。补充全基因组关联研究。
结论:我们确定了与5个神经认知特征相关的9个起源染色体区域。在每个相关区域中,我们鉴定了单核苷酸多态性(SNP),至少在某种程度上,混合物信号并在Black的独立样品中进行复制测试,非西班牙裔白人,和西班牙裔/拉丁裔成年人相同或相似的神经认知测试。在9个关联中的3个中观察到优先SNP的独立复制的统计证据,在chr4q12、chr9p22.1和chr13q12.13。在所有基因座上,观察到的认知功能和痴呆的关联有很强的生物学支持,优先考虑基因,如KIT,涉及神经毒性蛋白的自噬清除以及肥大细胞和小胶质细胞介导的炎症;SLC24A2,涉及与学习和记忆相关的突触可塑性;和MTMR6,涉及磷酸肌醇脂类代谢。
BACKGROUND: We conducted admixture mapping and fine-mapping analyses to identify ancestry-of-origin loci influencing cognitive abilities.
METHODS: We estimated the association of local ancestry intervals across the genome with five neurocognitive measures in 7140 diverse Hispanic and Latino adults (mean age 55 years). We prioritized genetic variants in associated loci and tested them for replication in four independent cohorts.
RESULTS: We identified nine local ancestry-associated regions for the five neurocognitive measures. There was strong biological support for the observed associations to cognitive function at all loci and there was statistical evidence of independent replication at 4q12, 9p22.1, and 13q12.13.
CONCLUSIONS: Our study identified multiple novel loci harboring genes implicated in cognitive functioning and dementia, and uncovered ancestry-relevant genetic variants. It adds to our understanding of the genetic architecture of cognitive function in Hispanic and Latino adults and demonstrates the power of admixture mapping to discover unique haplotypes influencing cognitive function, complementing genome-wide association studies.
CONCLUSIONS: We identified nine ancestry-of-origin chromosomal regions associated with five neurocognitive traits. In each associated region, we identified single nucleotide polymorphisms (SNPs) that explained, at least in part, the admixture signal and were tested for replication in independent samples of Black, non-Hispanic White, and Hispanic/Latino adults with the same or similar neurocognitive tests. Statistical evidence of independent replication of the prioritized SNPs was observed for three of the nine associations, at chr4q12, chr9p22.1, and chr13q12.13. At all loci, there was strong biological support for the observed associations to cognitive function and dementia, prioritizing genes such as KIT, implicated in autophagic clearance of neurotoxic proteins and on mast cell and microglial-mediated inflammation; SLC24A2, implicated in synaptic plasticity associated with learning and memory; and MTMR6, implicated in phosphoinositide lipids metabolism.