背景:母体抗乙型肝炎表面抗原(HBsAb)的抗体通过胎盘获得影响婴儿对乙型肝炎疫苗(HBVac)的免疫反应的程度仍不确定。目的:探讨HBsAb对小鼠HBVac免疫应答的影响。方法:根据注射的HBVac(2,5μg)的剂量,将267只BALB/c小鼠分为两组。根据给予的乙型肝炎免疫球蛋白(HBIG)(0、25、50IU)的剂量,将每组细分为3个亚组。在完成HepB疫苗接种后4周检测HBsAb滴度。结果:在所有小鼠中,40的HBsAb滴度<100mIU/mL(对HBVac无反应或低反应)。在0,25和50IUHBIG组中,HBsAb滴度<100mIU/mL的比率为1.1%,23.1%,和20.7%,分别。多因素logistic回归分析显示,HBVac低反应或无反应的危险因素为注射HBIG,低HBVac剂量,皮下注射。平均HBsAb滴度(log10)在0、25和50IUHBIG组中逐渐降低(P<0.001)。结论:HBIG给药对HBsAb的峰值水平和有效免疫应答率具有负面影响。这意味着通过胎盘获得的母体HBsAb可能会抑制婴儿对HBVac的免疫反应。
The extent to which maternal antibodies against the hepatitis B surface antigen (HBsAb) acquired transplacentally affect the immune responses to the hepatitis B vaccine (HBVac) in infants is still uncertain.
To explore the impact of the HBsAb on the immune response to the HBVac in a mouse model.
According to the doses of the HBVac (2, 5 μg) injected, 267 BALB/c mice were divided into two groups. Each group was subdivided into 3 subgroups based on the doses of the hepatitis B immunoglobulin (HBIG) (0, 25, 50 IU) administered. The HBsAb titers were detected 4 weeks after completing the HepB vaccination.
Among all the mice, 40 had an HBsAb titer <100 mIU/mL (non- or low-response to the HBVac). The rates of the HBsAb titer <100 mIU/mL in 0, 25 and 50 IU HBIG groups were 1.1%, 23.1%, and 20.7%, respectively. Multivariate logistic regression analysis showed that the risk factors for low- or non-response to the HBVac were injection with the HBIG, low HBVac dose, and hypodermic injection. The mean HBsAb titers (log10) reduced gradually in the 0, 25 and 50 IU HBIG groups (P<0.001).
The HBIG administration has negative impacts on the peak level of the HBsAb and the rate of an effective immune response. This implies that the maternal HBsAb acquired transplacentally might inhibit the immune responses to the HBVac in infants.