棉花被认为是巴基斯坦农业部门的主要作物。近年来,该地区的缺水降低了棉花高产的机会。选育棉花高产品种,即使在缺水的情况下,是当今的紧迫任务之一。为此,在随机完整区组设计中,在正常和水分亏缺条件下,以一式三份排列的方式筛选了40种不同基因型的陆地棉。在两种水情下,所有基因型均显示出显着差异。筛选出10个陆地棉耐水分亏缺(VH-144,IUB-212,MNH-886,VH-295,IR-3701,AA-802,NIAB-111,NS-121,FH-113和FH-142)和5个水分亏缺敏感(IR-3,CIM-443,FH-1000,MNH-147和S-12)。这些耐受和敏感的基因型在线×测试仪交配设计中杂交。为了进一步评估遗传物质,在下一个棉花生长季节,将50个F1杂交的种子及其15个亲本在正常和缺水条件下田间种植。研究中与产量相关的性状显示,种质及其半同胞之间存在显着差异。主成分分析(PCA)结果表明,因子1和因子2表现出的总变异分别为55.55%和41.95%,分别。PCA将变量转换为三个因素,只有两个因子(F1和F2)的特征值>1。优势程度表明,在两种水分条件下,所有参数都受到非加性基因作用的高度影响。此外,在水分亏缺胁迫下,品系VH-295和TesterCIM-443具有更好的产量表现。交叉组合,viz.,VH-144×S-12,NIAB-111×IR-3和VH-295×MNH-147对产量贡献性状最好。这些组合可能有助于在缺水情况下大规模提高种质资源。所有研究的性状都具有非加性类型的基因作用,表明这些基因型在棉花杂种开发计划中的用途,以对抗水分亏缺。
Cotton is considered as the main crop in the agricultural sector of Pakistan. Water deficiency in this region in recent years has reduced the chances of high yields of cotton. Selection and creation of high-yielding varieties of cotton, even in water deficit conditions, is one of urgent tasks of today. For this purpose, 40 diverse genotypes of upland cotton were screened in normal and water deficit conditions in triplicate arrangement under split plot in a randomized complete block design. All the genotypes showed significant difference under both water regimes. Ten upland cotton accessions were screened out as water deficit tolerant (VH-144, IUB-212, MNH-886, VH-295, IR-3701, AA-802, NIAB-111, NS-121, FH-113, and FH-142) and five as water deficit sensitive (IR-3, CIM-443, FH-1000, MNH-147, and S-12) based on seed cotton yield and stress susceptibility index. These tolerant and sensitive genotypes were crossed in line × tester mating design. For further evaluation of genetic material, the seed of 50 F1 crosses and their 15 parents were field planted under normal and water deficit conditions during next cotton growing season. Traits related to yield under the study showed significant variations among the accessions and their half sibs. The results of the principal component analysis (PCA) exhibited that total variation exhibited by factors 1 and factor 2 were 55.55 and 41.95%, respectively. PCA transformed the variables into three factors, and only two factors (F1 and F2) had eigenvalue > 1. The degree of dominance revealed that all parameters were highly influenced by non-additive gene action under both water regimes. Furthermore, the line VH-295 and tester CIM-443 had better yield performance under water deficit stress. The cross-combinations, viz., VH-144 × S-12, NIAB-111 × IR-3, and VH-295 × MNH-147, were the best for yield contributing traits. These combinations may be helpful for germplasm enhancement on large scale under water scarcity. All the studied traits have non-additive types of gene action suggesting the usage of these genotypes in cotton hybrid development program against water deficit tolerance.