在这项研究中,我们旨在通过整合的生物信息学分析和功能分析来确定调节鼻咽癌(NPC)进展的潜在靶点.NPC和正常组织样品之间的差异表达基因(DEGs)从基因表达综合(GEO)数据库中公开可用的微阵列数据集(GSE68799、GSE34573和GSE53819)获得。生物信息学分析从三个GEO数据集中确定了49个常见的DEG,主要富集在细胞因子/趋化因子通路和细胞外基质组织通路。进一步的蛋白质-蛋白质相互作用网络分析从49个DEG中鉴定出11个hub基因。通过分析Oncomine数据库,当与正常组织相比时,11个hub基因在NPC组织中显著上调。8个hub基因包括COL5A1、COL7A1、COL22A1、CXCL11、IFI44L、通过使用Oncomine数据库,当与正常组织相比时,在NPC组织中IFIT1、RSAD2和USP18显著上调。进一步的验证研究表明,IFIT1在NPC细胞中上调。IF1T1的击倒抑制了增殖,迁移,和侵袭的鼻咽癌细胞;而IFIT1过表达促进增殖,迁移,和侵袭NPC细胞。总之,使用整合的生物信息学分析,共49个DEGs和11个hub基因在NPC中。IFIT1在NPC细胞系中上调,IFIT1可能通过促进NPC细胞增殖而作为癌基因,迁移,和入侵。
In this study, we aimed to identify potential targets modulating the progression of nasopharyngeal carcinoma (NPC) using integrated bioinformatics analysis and functional assays. Differentially expressed genes (DEGs) between NPC and normal tissues samples were obtained from publicly availably microarray datasets (GSE68799, GSE34573, and GSE53819) in the Gene Expression Omnibus (GEO) database. The bioinformatics analysis identified 49 common DEGs from three GEO datasets, which were mainly enriched in cytokine/chemokine pathways and extracellular matrix organization pathway. Further protein-protein interaction network analysis identified 11 hub genes from the 49 DEGs. The 11 hub genes were significantly up-regulated in the NPC tissues when compared to normal tissues by analyzing the Oncomine database. The 8 hub genes including COL5A1, COL7A1, COL22A1, CXCL11, IFI44L, IFIT1, RSAD2, and USP18 were significantly up-regulated in the NPC tissues when compared to normal tissues by using the Oncomine database. Further validation studies showed that IFIT1 was up-regulated in the NPC cells. Knockdown of IFI1T1 suppressed the proliferation, migration, and invasion of NPC cells; while IFIT1 overexpression promoted the proliferation, migration, and invasion of NPC cells. In conclusion, a total of 49 DEGs and 11 hub genes in NPC using the integrated bioinformatics analysis. IFIT1 was up-regulated in the NPC cells lines, and IFIT1 may act as an oncogene by promoting NPC cell proliferation, migration, and invasion.