封装在壳聚糖(CS)微胶囊(IA/CS)中的IturinA(IA)使用热重分析(TGA)进行了彻底的物理化学表征,傅里叶变换红外(FTIR)光谱,和扫描电子显微镜(SEM)。SEM证实了光滑,IA/CS微胶囊的球形形态,而FTIR揭示了IA和CS之间复杂的分子间相互作用。TGA在0-100°C范围内表现出热稳定性,而粒度分析显示平均直径为553.4nm。评估IA/CS在葡萄采后保鲜中的功效,用无菌水(CK)处理葡萄,10g/LCS,0.1g/LIA/CS,和0.1g/L壳聚糖空微胶囊(CKM),然后在25°C下储存16天。IA/CS显著降低衰变和呼吸强度52.3%和23.8%,分别,相比于CK。IA/CS治疗也抑制脱落率,减肥,硬度降低,可溶性固形物总消耗量,可滴定酸度消耗,多酚氧化酶,过氧化物酶活性与CS处理相当(p>0.05),但表现优于CK(减少26.9%,41.2%,25.8%,27.2%,24.2%,19.4%,和17.4%,分别)和CKM(p<0.05)。感官评估证实,IA/CS有效抑制腐烂,采后代谢活动减慢,保持葡萄品质。因此,IA/CS微胶囊为延长葡萄保质期和保持品质提供了一种有前途的方法。
Iturin A (IA) encapsulated in chitosan (CS) microcapsules (IA/CS) underwent thorough physicochemical characterization using thermogravimetric analysis (TGA), Fourier-transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). SEM confirmed the smooth, spherical morphology of the IA/CS microcapsules, while FTIR revealed complex intermolecular interactions between IA and CS. TGA demonstrated thermal stability within the 0-100 °C range, while particle size analysis revealed an average diameter of 553.4 nm. To evaluate IA/CS efficacy in post-harvest grape preservation, grapes were treated with sterile water (CK), 10 g/L CS, 0.1 g/L IA/CS, and 0.1 g/L chitosan empty microcapsules (CKM), then stored at 25 °C for 16 days. IA/CS significantly reduced decay and respiration intensity by 52.3 % and 23.8 %, respectively, compared to CK. IA/CS treatment also inhibited abscission rate, weight loss, firmness reduction, total soluble solids consumption, titratable acidity consumption, polyphenol oxidase, and peroxidase activities on par with CS treatment (p > 0.05), but performed better than CK (reductions of 26.9 %, 41.2 %, 25.8 %, 27.2 %, 24.2 %, 19.4 %, and 17.4 %, respectively) and CKM (p < 0.05). Sensory evaluation confirmed that IA/CS effectively suppressed decay, slowed post-harvest metabolic activity, and maintained grape quality. Therefore, IA/CS microcapsules offer a promising method for extending grape shelf life and preserving quality.