在动物实验中实现手术麻醉,选择合适的麻醉剂量很重要。然而,很少有研究调查树sh(Tupaiabelangeri)的合理麻醉剂量。该研究的目的是回顾文献,以确定树the中最常用的麻醉剂量,并根据体表面积换算计算树the和大鼠之间的合理等效剂量。两组分别以62mg/kg(等效剂量)和40mg/kg(报告剂量)的剂量分别用1%戊巴比妥钠通过腹膜内注射麻醉,每组10只成年树the。除生命体征外,还评估了麻醉深度和时间。结果表明,不同研究的剂量差异很大,范围从15mg/kg到80mg/kg,40mg/kg是最常见的报告剂量。然而,用通常报道的剂量麻醉的树sh组无法满足手术要求。相比之下,等效剂量(62mg/kg,通过体表面积转换计算,腹膜内注射戊巴比妥钠)可以达到44.28±3.95分钟的麻醉时间,没有严重或致命的影响。在麻醉监测期间,我们发现戊巴比妥钠对血压有抑制作用,脉搏率,树sh的呼吸频率和直肠温度,尤其是呼吸频率。因此,我们的研究表明,使用等效剂量的戊巴比妥钠可以有效地麻醉树。
To achieve surgical anesthesia in animal experimentation, it is important to select the appropriate anesthetic dose. However, few studies have investigated the reasonable anesthetic dose in tree shrew (Tupaia belangeri). The aim of the study was to review the literature to determine the most commonly used anesthetic dose in tree shrew and to calculate the reasonable equivalent dose between tree shrew and rat based on the body surface area conversion. Two groups of 10 adult tree shrews each were anesthetized with 1% sodium pentobarbital through intraperitoneal injection separately at doses of 62 mg/kg (equivalent dose) and 40 mg/kg (reported dose). Anesthetic depth and times were assessed in addition to vital signs. The results showed that the dosage was quite different across studies, ranging from 15 mg/kg to 80 mg/kg, with 40 mg/kg being the most frequently reported dose. However, the group of tree shrews anesthetized with the commonly reported dose were unable to meet the requirements of surgery. In contrast, the equivalent dose (62 mg/kg, intraperitoneal injection with sodium pentobarbital) calculated by body surface area conversion could achieve an anesthetic time of 44.28 ± 3.95 min with no serious or fatal effects. During anesthetic monitoring, we found that sodium pentobarbital had an inhibitory effect on the blood pressure, pulse rate, respiratory rate and rectal temperature in tree shrews, especially on the respiratory rate. Thus, our study indicated that the use of the equivalent dose of sodium pentobarbital was effective in anesthetizing tree shrews.