蛋壳是鸡蛋品质的重要指标之一,由于外壳强度低,痘痘蛋(PE)更容易破损,从而给鸡蛋行业造成巨大的经济损失。目前,调节痘痘卵形成的分子机制知之甚少。在这项研究中,通过全转录组测序分析PE蛋鸡(n=8)和正常卵(NE)蛋鸡(n=8)的子宫组织,和总共619个差异表达的mRNA(DEmRNA),获得122个差异表达的lncRNA(DElncRNA)和21个差异表达的miRNA(DEmiRNA)。基于DEmRNA之间的靶向关系,DElncRNAs和DEmiRNAs,我们构建了一个竞争性内源性RNA(ceRNA)网络,包括12个DEmiRNA,19DElncRNAs,和128个DEmRNA。考虑到网络中包含的大量信息,我们构建了一个更小的ceRNA网络,以更好地理解丘疹卵形成的复杂机制。较小的ceRNA网络包含7个DElncRNAs(LOC107056551,LOC121109367,LOC121108909,LOC121108862,LOC112530033,LOC121113165,LOC107054145),5个DEmiRNA(gga-miR-6568-3p,gga-miR-31-5p,gga-miR-18b-3p,gga-miR-1759-3p,gga-miR-12240-3p)和7个DEmRNA(CABP1,DNAJC5,HCN3,HPCA,IBSP,KCNT1,OTOP3),这些差异表达的基因可能在母鸡丘疹卵的形成中起着关键的调控作用。这项研究提供了mRNAs的整体表达谱,母鸡子宫组织中的lncRNAs和miRNAs,为进一步研究丘疹卵形成的分子机制提供了理论依据,在提高蛋壳质量方面具有潜在的应用价值。
Eggshell is one of the most important indicators of egg quality, and due to low shell strength, pimple eggs (PE) are more susceptible to breakage, thus causing huge economic losses to the egg industry. At the current time, the molecular mechanisms that regulate the formation of pimple eggs are poorly understood. In this study, uterine tissues of PE-laying hens (n = 8) and normal egg (NE) -laying hens (n = 8) were analyzed by whole transcriptome sequencing, and a total of 619 differentially expressed mRNAs (DE mRNAs), 122 differentially expressed lncRNAs (DE lncRNAs) and 21 differentially expressed miRNAs (DE miRNAs) were obtained. Based on the targeting relationship among DE mRNAs, DE lncRNAs and DE miRNAs, we constructed a competitive endogenous RNA (ceRNA) network including 12 DE miRNAs, 19 DE lncRNAs, and 128 DE mRNAs. Considering the large amount of information contained in the network, we constructed a smaller ceRNA network to better understand the complex mechanisms of pimple egg formation. The smaller ceRNA network network contains 7 DE lncRNAs (LOC107056551, LOC121109367, LOC121108909, LOC121108862, LOC112530033, LOC121113165, LOC107054145), 5 DE miRNAs (gga-miR-6568-3p, gga-miR-31-5p, gga-miR-18b-3p, gga-miR-1759-3p, gga-miR-12240-3p) and 7 DE mRNAs (CABP1, DNAJC5, HCN3, HPCA, IBSP, KCNT1, OTOP3), and these differentially expressed genes may play key regulatory roles in the formation of pimpled eggs in hens. This study provides the overall expression profiles of mRNAs, lncRNAs and miRNAs in the uterine tissues of hens, which provides a theoretical basis for further research on the molecular mechanisms of pimpled egg formation, and has potential applications in improving eggshell quality.