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Abstract：
Hatchability could be quite different among individuals of indigenous chicken breed which might be affected by the egg quality. In this study, hatchability was individually recorded among 800 forty-wk-old Huainan partridge chickens. The chickens were then divided into high and low hatchability groups (HH and LH group) with 50 birds in each group. Egg quality was further determined in the 2 groups. Eight birds from each group were selected for slaughtering and tissue, responsible for egg formation, collection for structure observation by staining and candidate gene expression by transcriptome analysis. The hatchability in HH was 100% and 61.18% in LH. The eggshell thickness and shell strength were significantly lower, while the albumen height and Haugh unit were significantly higher in HH group than those in LH group (P < 0.05). The magnum weight and index, and the expression of polypeptide N-acetylgalactosaminyltransferase 9 (GALNT9), which responsible for thick albumen synthesis, in HH group were also significantly higher than that of LH group (P < 0.05). Compared with the LH group, there were 702 differentially expressed genes (DEGs) in HH group, of which 402 were up-regulated and 300 were down-regulated. Candidate genes of calbindin 1 (CALB1) and solute carrier family 26 member 9 (SLC26A9), which regulate calcium signaling pathway so as to affect Ca2+ transportation, exhibited significant high and low expression, respectively, in HH group compared to those in LH group (P < 0.05). Therefore, indigenous chicken with high expression of GALNT9 in magnum to form thick albumen to provide more protein for embryo, while high CALB1 and low expression of SLC26A9 to decrease Ca2+ transportation so as to form a thinner eggshell and provide better gas exchange during embryo development.
摘要：
本地鸡品种的孵化率可能大不相同，这可能会受到鸡蛋质量的影响。在这项研究中,800只40周龄淮南part鸡分别记录了孵化率。然后将鸡分为高孵化率和低孵化率组(HH和LH组),每组50只。进一步测定2组的蛋品质。每组选取8只鸟进行屠宰和组织,负责卵的形成，通过染色进行结构观察和通过转录组分析进行候选基因表达。HH的孵化率为100％，LH的孵化率为61.18％。蛋壳厚度和壳强度明显降低，HH组蛋白高度和Haugh单位明显高于LH组（P<0.05）。大号权重和指数，和多肽N-乙酰氨基半乳糖转移酶9（GALNT9）的表达，负责粗蛋白合成，HH组也明显高于LH组(P<0.05)。与LH组相比，HH组中有702个差异表达基因(DEGs),其中402个上调，300个下调。钙结合蛋白1（CALB1）和溶质载体家族26成员9（SLC26A9）的候选基因，调节钙信号通路从而影响Ca2+的运输,表现出显著的高表达和低表达，分别,HH组与LH组比较(P<0.05)。因此,高表达GALNT9的土鸡能形成厚蛋白，为胚胎提供更多的蛋白质，而高CALB1和SLC26A9的低表达减少了Ca2的运输，从而形成更薄的蛋壳并在胚胎发育过程中提供更好的气体交换。
