背景:唐古特大黄(Rh。tangutum)是一种重要的中药植物,\"大煌\",其中含有生产性代谢产物,并在青藏高原上占据广泛的栖息地。占据广泛生境的植物通常在形态和代谢等表型上有所不同,从而发展成不同的生态型。在冠层和开放的栖息地下是一对具有Rh的不同栖息地。唐古树植物。然而,很少有研究关注栖息地对Rh的影响。唐古菜生长,特别是结合形态和代谢变化。本研究集中在Rh。在冠层和开放生境下生长的tangutum植物,使用非目标代谢方法对形态和代谢变化进行了定量。
结果:获得的结果表明,两个不同的生境导致Rh。唐古汀发育成两种不同的生态型,其形态和代谢同时发生改变。在冠层栖息地下繁殖形态较小的Rh。Tangutum植物具有较高水平的代谢物(31个中的22个),其中包括五种类黄酮,四种异黄酮,和三个蒽.另一方面,开放的栖息地产生了形态上较大的Rh。具有较高水平的代谢物(31个中的9个)(包括四种类黄酮)的唐古菜植物。预测31种代谢物中有6种具有效应靶标,包括4个代表树冠下的栖息地,2个代表开放的栖息地。完全正确,208个目标连接,其中42个是树冠下和开放栖息地代表化合物的公共目标,100和66是树冠下优越化合物和开放栖息地优越化合物的独特目标,分别。此外,芦荟大黄素,大黄素,大黄酚,physcion,皂甙A和皂甙B都更多地积累在冠层生境下,其中芦荟大黄素,大黄素,在冠层生境下,大黄酚和physcion明显较高。
结论:本研究确定Rh。在冠层下和开放生境中生长的唐古菜发展成两种不同的生态型,具有形态和代谢差异。网络药理学研究结果表明,“大黄”来自不同的栖息地,例如在树冠和开放的栖息地下,是不同的效果目标,因此可能有不同的药用。根据目标代谢组学,在冠层栖息地下,“大黄”可能会生长得更好。
BACKGROUND: Rheum tanguticum (Rh. tanguticum) is an important traditional Chinese medicine plant, \"Dahuang\", which contains productive metabolites and occupies wide habitats on the Qinghai-Tibet plateau. Plants occupying wide habitats usually vary in phenotypes such as in morphology and metabolism, thereby developing into different
ecotypes. Under canopy and open habitats are a pair of dissimilar habitats which possess Rh. tanguticum plants. However, few studies have focused on the effect of habitats on Rh. tanguticum growth, particularly combining morphological and metabolic changes. This study focused on Rh. tanguticum plants growing in under canopy and open habitats where morphology and metabolism changes were quantified using non-target metabolism methods.
RESULTS: The obtained results indicated that the two dissimilar habitats led to Rh. tanguticum developing into two distinct
ecotypes where the morphology and metabolism were simultaneously changed. Under canopy habitats bred morphologically smaller Rh. tanguticum plants which had a higher level of metabolites (22 out of 31) which included five flavonoids, four isoflavonoids, and three anthracenes. On the other hand, the open habitats produced morphologically larger Rh. tanguticum plants having a higher level of metabolites (9 out of 31) including four flavonoids. 6 of the 31 metabolites were predicted to have effect targets, include 4 represent for under canopy habitats and 2 for open habitats. Totally, 208 targets were connected, among which 42 were communal targets for both under canopy and open habitats represent compounds, and 100 and 66 were unique targets for under canopy superior compounds and open habitats superior compounds, respectively. In addition, aloe-emodin, emodin, chrysophanol, physcion, sennoside A and sennoside B were all more accumulated in under canopy habitats, and among which aloe-emodin, emodin, chrysophanol and physcion were significantly higher in under canopy habitats.
CONCLUSIONS: This study determined that Rh. tanguticum growing in under canopy and in open habitats developed into two distinct
ecotypes with morphological and metabolic differences. Results of network pharmacology study has indicated that \"Dahuang\" coming from different habitats, such as under canopy and open habitats, are different in effect targets and thus may have different medicinal use. According to target metabolomics, under canopy habitats may grow better \"Dahuang\".