The rapid cultivation of partial nitritation/ANAMMOX （PN/A） granular sludge in a continuous-flow mode is one of the key technologies for efficient biological nitrogen removal in domestic wastewater treatment. Compared with that in PN/A granular sludge, PN granular sludge demonstrates a shorter incubation period and suitability for batch culture. It is also a good carrier for enriching ANAMMOX （AMX） bacteria. In this study, we established a continuous-flow autotrophic nitrogen removal process in three continuously stirred tank reactors （CSTR） （R1-R3） by hybrid-inoculating PN/A and PN granular sludge at the mass ratios of 3∶1, 1∶1, and 1∶3, respectively. By implementing high ammonium nitrogen loading and short hydraulic retention time, continuous autotrophic nitrogen removal processes were successfully started up in the three CSTRs. The results showed that compared with that of R1 and R2, R3 had a longer start-up time but a similar steady-state nitrogen removal performance. The total nitrogen removal load of R3 could be more than 2.6 kg·（m3·d）-1. Intriguingly, the inoculated PN granular sludge served as a precursor for PN/A granular sludge cultivation. This approach facilitated the enrichment of anaerobic ammonia-oxidizing bacteria （AMX） by introducing abundant ammonium-oxidizing bacteria （AOB） and nitrite nitrogen substrates into the CSTR. According to the results of high-throughput sequencing, the microbial abundance and diversity of the mature granules in R1-R3 were significantly higher than those of the inoculation sludge. AOB （genus Nitrosomonas）, AMX （genera Candidatus Kuenenia and Candidatus Brocadia）, and symbiotic heterotrophs, such as Chloroflexi, Bacteroidetes, and Chlorobi, drove the autotrophic nitrogen removal process and maintained the stability of the granular structure. In summary, a novel start-up strategy of hybrid-inoculating granular sludge was provided for a continuous-flow autotrophic nitrogen removal in engineering application.

Achieving economic and efficient removal of nutrients in mainstream wastewater treatment plants (WWTPs) continues to be a challenging research topic. In this study, a continuous-flow anaerobic/aerobic/anoxic system with sludge double recirculation (AOA-SDR), which integrated partial nitrification (PN), endogenous denitrification (ED) and nitrite-type denitrifying phosphorus removal (nDNPR), was constructed to treat real carbon-limited municipal wastewater. The average effluent concentrations of total inorganic nitrogen (TIN) and PO43--P during the stable operation period were 1.8 and 0.3 mg/L, respectively. PN was achieved with an average nitrite accumulation ratio of 90.4 % by combined strategies. Adequate storage of polyhydroxyalkanoates and glycogen in the anaerobic zone promoted the subsequent nitrogen removal capacity. In the anoxic zone, nitrite served as the main electron acceptor for the denitrifying phosphorus removal process. Mass balance analysis revealed that nDNPR contributed to 23.6 % of TIN removal and 44.7 % of PO43--P removal. The enrichment of Nitrosomonas (0.45 %) and Ellin 6067 (1.31 %), along with the washout of Nitrospira (0.15 %) provided the bacterial basis for the successful implementation of PN. Other dominant endogenous heterotrophic bacteria, such as Dechlormonas (10.81 %) and Candidatus Accumulibacter (2.96 %), ensured simultaneous nitrogen and phosphorus removal performance. The successful validation of integrating PN, ED and nDNPR for advanced nutrient removal in the AOA-SDR process provides a transformative technology for WWTPs.

Anthraquinone dyes are the second most important type of dyes after azo dyes. In particular, 1-aminoanthraquinone has been extensively utilized in the preparation of diverse anthraquinone dyes. This study employed a continuous-flow method to synthesize 1-aminoanthraquinone safely and efficiently through the ammonolysis of 1-nitroanthraquinone at high temperatures. Various conditions (reaction temperature, residence time, molar ratio of ammonia to 1-nitroanthraquinone (M-ratio), and water content) were investigated to explore the details of the ammonolysis reaction behavior. Operation conditions for the continuous-flow ammonolysis were optimized using Box-Behnken design in the response surface methodology, and ~88% yield of 1-aminoanthraquinone could be achieved with an M-ratio of 4.5 at 213 °C and 4.3 min. The developed process\'s reliability was evaluated by performing a 4 h process stability test. The kinetic behavior for the preparation of 1-aminoanthraquinone was investigated under continuous-flow mode to guide the reactor design and to gain a deeper understanding of the ammonolysis process.

Currently, there is a lack of advanced nanotechnology designed to efficiently remove antimony (Sb) from contaminated water systems. Sb most commonly appears as antimonite (Sb(III)) or as the anion antimonate (Sb(V)). Sb(III) is approximately ten times more toxic than Sb(V), and Sb(III) is also harder to eliminate because of its motility and charge neutrality. The work presented here developed an electrochemical filtration technology for the direct elimination of Sb(III) from contaminated water. The primary components of the filtration system were an electroactive carbon nanotube (CNT) membrane that were functionalized with the Sb-specific UiO-66(Zr), an metal-organic framework. In an electric field, the UiO-66(Zr)/CNT nanohybrid filter enabled in situ transformation of Sb(III) to less harmful Sb(V). The Sb(V) was then effectively adsorbed by the UiO-66(Zr). The removal efficiency (90.5%) and rate constant (k1 = 0.0272 min-1) toward Sb(III) removal was 1.3 and 1.4 times greater than that of CNT filter. The abundance of available adsorption sites of the nanohybrid filter, flow-through construction, and electrochemical activity combined to rapidly remove Sb(III) from water. The underlying functioning of the nanohybrid filter was determined with a series of process experiments and structural characterizations. The filter was effective over a broad range of pH values and in a variety of complex aqueous environments. Once loaded with Sb, the UiO-66(Zr)/CNT filter could be washed with a dilute NaOH solution to efficiently refresh its activity. The results of this work offer a direct, efficient strategy that integrates nanotechnology, electrochemistry, and membrane separation to remove antimony and potentially other heavy metals from contaminated water.

Microwave continuous-flow liquid food sterilisation, in which the liquid is mainly heated by microwaves, has the advantages of fast sterilisation speed, energy saving, comprehensive elimination, and less nutrient loss. Circular pipes are commonly used in microwave continuous-flow liquid heating processing. However, with circular pipes, which are widely used in the industry, the heating is uneven owing to the phenomenon of tube focusing when adopting external radiation. In this study, a novel microwave continuous-flow milk sterilisation system based on a coaxial slot radiator is proposed. First, the coaxial slot radiator was designed to realise efficient radiation through the establishment of multi-physics model. The structure of the system was then optimised by comparing the heating efficiency and uniformity of simulation results. The effect of microwave coaxial slot radiator rotation on heating uniformity was simulated and the results show that the heating uniformity is improved obviously. Experimental equipment was set up to verify the results of the simulation. The experimental results are consistent with the simulation results. Finally, the sensitivity analysis of the system is performed to confirm that, when the dielectric properties and types of liquid food change, the heating of the proposed microwave continuous-flow system remains efficient and uniform.

An efficient continuous-flow nitration process of o-xylene at pilot scale was demonstrated. The effects of parameters such as temperature, ratio of H2SO4 to HNO3, H2SO4 concentration, flow rate, and residence time on the reaction were studied. Under the optimal conditions, the yield of products reached 94.1%, with a product throughput of 800 g/h. The main impurities of this continuous-flow nitration process were also studied in detail. Compared with batch process, phenolic impurity decreased from 2% to 0.1%, which enabled the omission of the alkaline solution washing step and thus reduced the wastewater emission. The method was also successfully applied to the nitrification of p-xylene, toluene, and chlorobenzene with good yields.

Phosphorus (P) has been identified as a major cause of eutrophication. One feasible way to deal with P-containing wastewater is to employ advanced adsorbents with high P affinity. Towards this end, the loading of these sorbents onto a conductive scaffold would facilitate the introduction of an electric field into the reaction system thereby permitting a continuous-flow operation and improved P sorption kinetics. Here, the preparation and evaluation of an electroactive carbon nanotube (CNT) filter functionalized with cerium-based metal organic frameworks (Ce-MOF) is reported. Various advanced characterization techniques confirmed the successful fabrication of the Ce-MOF/CNT nanohybrid filter. The results suggested that the nanohybrid filter had a maximum P adsorption capacity of 22.41 mg g-1, which compared favorably with other state-of-the-art P sorbents. Ce-MOF loading, applied voltage and flow rate each increased the rate constants for phosphate removal by factors of 1.6, 2.1 and 5.8 times relative to the absent states. The underlying P sorption mechanisms involved outer-sphere surface complexation (electrostatic attraction), inner-sphere surface complexation (Ce-O-P) and diffusion. The performance was tolerant of a wide operational pH range and different water matrices. The Ce-MOF/CNT electrochemical filter described in this study provides a viable strategy to address the challenging issues associated with aqueous P pollution.

A U-type membraneless continuous-flow bioelectrochemical system was developed to efficiently remove tetracycline and antibiotic resistance genes from synthetic wastewaters at hydraulic retention time of only eight hours. At the TC concentration of 20-80 mgL-1 in feed, the removals of tetracycline all exceeded 95%, over 60-1200 mgL-1 chemical oxygen demand, 30-150 mgL-1 NH4+-N, and at 5-25 °C, superior to the performances reported in literature. The maximum power of the BES system peaked at 0.416 Wm-3 at 20 mgL-1 TC feeding, corresponding to open circle voltage of 0.90 V and internal resistance of 799.8 Ω. The community analysis showed that the elevated TC loadings forced the predominate population to be evolved to TC-degrading consortium. The relative abundances of tetA, tetC, tetO, tetQ, and tetW in treated effluent ranged 1.20 × 10-6 to 2.60 × 10-4, revealing that the present BES reactor has superior removal efficiency of antibiotic resistance genes.

In this study, a continuous-flow bioreactor packed with well-organized lipase microarrays was developed for the sustainable synthesis of functional lipid-phytosterol esters (PEs). Hollow mesoporous silicon spheres with a suitable pore size were prepared for lipase immobilization, and the hydrophobic modification endowed the lipase with excellent catalytic activity and stability. The results showed that the condensely packed lipase microarrays offered large specific surface areas and guaranteed the thorough interaction between the lipase and substrates in the continuous-flow bioreactor. Meanwhile, the substrate could pass through the reactor at 1 mL/min with a high conversion of 93.6% due to the hollow structure of the packing spheres. Moreover, the reactors were able to produce 1564 g PEs/g catalyst in a continuous 30-day processing period, which set the highest records for PEs synthesis. This sustainable and highly-converting flow system provided a feasible path for scale-up production of PEs in the food processing area.

A novel porous ceramic sheet supported nickel particles reactor was obtained by an in-situ preparation method. This reactor was then used to investigate continuous-flow catalysis of nitroaromatic compounds and methyl orange. The details of the structure and morphology were characterized by XRD, SEM, XPS, Raman, element mapping, mercury intrusion method and Archimedes principle. The porous ceramic sheet supported Ni particles reactor exhibited excellent catalytic performance in the catalytic reduction of p-nitrophenol and methyl orange by sodium borohydride at room temperature. Both the conversion of p-nitrophenol (5 mM) and methyl orange (0.3 mM) reached nearly 100% at the injection speed of 2.67 mL·min-1. In addition, it maintained conversions of 100% after 10 recycling time since the porous ceramic sheet could reduce the aggregation for Ni particles. Furthermore, the chemisorbed oxygen, and the strong interaction between Ni and porous ceramic sheet resulted in a highly efficient, recoverable, and cost-effective multifunctional reactor. All of these advantages present new opportunities to be implemented in the field of waste water treatment and environmental toxicology. Ultimately, the porous ceramic sheet could also support other metal nanomaterial, and used in other fields of environmental catalysis.