■牙槽裂(AC)是唇腭裂(CLP)患者常见的先天性缺陷。牙槽骨移植(ABG)通常在青春期进行,导致裂隙在口腔中保留更长的时间。AC患者患龋齿等口腔疾病的比例高于正常人群,由AC引起的细菌改变的确切特征是未知的。
■我们共招募了87名受试者,并从AC青少年(AAP)收集了牙菌斑样本,术后青少年ABG(PAP),健康控制青少年(CAP),AC年轻人(AYP),术后ABG年轻人(PYP),和健康控制年轻人(CYP)。对16SrRNA基因进行测序。
■牙槽沟裂患者斑块的微生物组成与年龄匹配的健康对照有显著差异。线性判别分析效应大小(LEfSe)分析表明,AAP对奈瑟菌、嗜血杆菌,梭杆菌,红球菌,Aggregatibacter,Gemella,和卟啉虫,而AYP富含Capnocytophaga,红球菌,和放线菌-f0332。兼性厌氧存在表型差异,革兰氏阴性,革兰氏阳性,根据Bugbase表型预测,具有较长肺泡裂隙的AYP组和健康对照组之间的氧化应激耐受性。牙槽骨移植并未改变牙槽沟裂患者的功能表型,但减少了两个年龄段的牙槽沟裂患者和健康对照者之间的差异属数量。
■我们的研究系统地表征了牙槽裂患者的牙龈上斑块微生物群,牙槽骨移植术后患者,与两个年龄段的健康对照进行匹配,以更好地了解与肺泡裂隙相关的斑块生态学和微生物学。
UNASSIGNED: Alveolar cleft (AC) is a common congenital defect in people with cleft lip and palate (CLP). Alveolar bone grafting (ABG) is typically performed during adolescence, resulting in the fissure remaining in the mouth for a longer length of time. Patients with AC have a greater rate of oral diseases such as dental caries than the normal population, and the precise characteristics of the bacterial alterations caused by AC are unknown.
UNASSIGNED: We recruited a total of 87 subjects and collected dental plaque samples from AC adolescents (AAP), post-operative ABG adolescents (PAP), healthy control adolescents (CAP), AC young adults (AYP), post-operative ABG young adults (PYP), and healthy control young adults (CYP). The sequencing of 16S rRNA genes was performed.
UNASSIGNED: The microbial composition of plaque from alveolar cleft patients differed significantly from age-matched healthy controls. Linear discriminant analysis effect size (LEfSe) analysis revealed that AAP was enriched for Neisseria, Haemophilus, Fusobacterium, Rhodococcus, Aggregatibacter, Gemella, and Porphyromonas, whereas AYP was enriched for Capnocytophaga, Rhodococcus, and Actinomyces-f0332. There were phenotypic differences in facultatively anaerobic, Gram-negative, Gram-positive, and oxidative stress tolerance between the AYP group with longer alveolar cleft and the healthy control group according to Bugbase phenotypic predictions. Alveolar bone grafting did not alter the functional phenotype of alveolar cleft patients but reduced the number of differential genera between alveolar cleft patients and healthy controls at both ages.
UNASSIGNED: Our study systematically characterized the supragingival plaque microbiota of alveolar cleft patients, post-alveolar bone grafting patients, and matched healthy controls in two ages to gain a better understanding of plaque ecology and microbiology associated with alveolar clefts.