未经批准:粘菌素是治疗耐碳青霉烯类肠杆菌(CREs)的少数选择之一。关于川南地区粘菌素耐药CREs的流行状况,现有资料很少,中国。本研究主要研究了从泸州分离的广泛耐药大肠杆菌LZ00114的基因组和表型特征,中国。
UNASSIGNED:2020年,从泸州一名肾积水尿路感染患者的尿液中分离出LZ00114,中国。我们通过抗菌素敏感性测试评估了在存在和不存在质子基团羰基氰基-间氯苯基肼(CCCP)和1-(1-萘基甲基)哌嗪(NMP)的情况下LZ00114的耐药性。生长动力学,运动性,并对LZ00114进行致病性测定,以评价其微生物特性。结合全基因组测序(WGS)和实时逆转录PCR(RT-PCR),我们全面分析了LZ00114的抗性机制。
未经证实:LZ00114对各种抗菌药物具有耐药性,包括美罗培南,四环素,环丙沙星,庆大霉素,磷霉素,和多粘菌素B值得注意的是,CCCP逆转了LZ00114对多粘菌素B的抗性。与Lab-WT菌株相比,LZ00114在感染模型中显示出高致病性(P<0.01)。其生长速率和运动能力与Lab-WT菌株没有显着差异。WGS和结合显示LZ00114属于ST410,并携带一个带有blaNDM-5的自传播性IncX3质粒和一个带有blaOXA-1-blaCTX-M-55-tet(B)-aac(6')-Ib-cr-dfrA17-sul1-fosA3的多复制子IncFII/FIA/FIB质粒。比较基因组学揭示了LZ00114与其他地区分离的菌株之间的遗传相关性。此外,pmrA有点突变(S29G,G144S),pmrB(D283G,Y358N),marR(G103S,Y137H),emrA(I219V),以及LZ00114的emrD(G323D)。RT-PCR证实了LZ00114中外排泵和PmrABC的过表达。
UNASSIGNED:本研究为监测抗菌素耐药性提供了有价值的信息,为预防和控制粘菌素耐药性大肠杆菌提供了理论依据。仍需警惕高危克隆群体大肠杆菌ST410的克隆流传。
UNASSIGNED: Colistin is one of the few options for treating carbapenem-resistant Enterobacterales (CREs). There is little available information about the epidemic status of colistin-resistant CREs in Southern Sichuan,
China. This study mainly investigated the genomic and phenotypic characteristics of an extensively drug resistant E. coli LZ00114 isolated from Luzhou,
China.
UNASSIGNED: In 2020, LZ00114 was isolated from the urine of a patient with hydronephrosis and urinary tract infection in Luzhou,
China. We assessed the resistance profile of LZ00114 in the presence and absence of the protonophore carbonyl cyano-m-chlorophenylhydrazine (
CCCP) and 1-(1-naphthylmethyl)-piperazine (NMP) by antimicrobial susceptibility testing. The growth kinetics, motility, and pathogenicity of LZ00114 were determined to evaluate its microbial characteristics. In combination with whole genome sequencing (WGS) and real-time reverse transcription PCR (RT-PCR), we comprehensively analyzed the resistance mechanisms of LZ00114.
UNASSIGNED: LZ00114 was resistant to various antimicrobial agents, including meropenem, tetracycline, ciprofloxacin, gentamicin, fosfomycin, and polymyxin B. Notably,
CCCP reversed the resistance of LZ00114 to polymyxin B. LZ00114 displayed high pathogenicity in the infection model (P<0.01) compared with the Lab-WT strain, and its growth rate and motility were not significantly different from the Lab-WT strain. WGS and conjugation revealed that LZ00114 belonged to ST410 and carried a bla NDM-5-harboring self-transmissible IncX3 plasmid and a multi-replicon IncFII/FIA/FIB plasmid carrying bla OXA-1-bla CTX-M-55-tet(B)-aac(6\')-Ib-cr-dfrA17-sul1-fosA3. Comparative genomics revealed genetic relatedness between LZ00114 and strains isolated from other regions. Furthermore, there were point mutations in pmrA (S29G, G144S), pmrB (D283G, Y358N), marR (G103S, Y137H), emrA (I219V), and emrD (G323D) of LZ00114. RT-PCR confirmed the overexpression of efflux pumps and PmrABC in LZ00114.
UNASSIGNED: This study provides valuable information for the surveillance of antimicrobial resistance and a theoretical basis for the prevention and control of colistin-resistant E. coli. There is still a need to be vigilant about the clone spread of the high-risk clone group E. coli ST410.