PDF(Pubmed)
Abstract：
Duck plague virus (DPV) is extremely infectious and lethal, so antiviral drugs are urgently needed. Our previous study shows that DPV infection with duck embryo fibroblast (DEF) induces reactive oxygen species (ROS) changes and promotes apoptosis. In this study, we tested the antiviral effect of the carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a common mitochondrial autophagy inducer. Our results demonstrated a dose-dependent anti-DPV effect of CCCP, CCCP-treatment blocked the intercellular transmission of DPV after infection, and we also proved that CCCP could have an antiviral effect up to 48 hpi. The addition of CCCP reversed the DPV-induced ROS changes, CCCP can inhibit virus-induced apoptosis; meanwhile, CCCP can affect mitochondrial fusion and activate mitophagy to inhibit DPV. In conclusion, CCCP can be an effective antiviral candidate against DPV.
摘要：
鸭瘟病毒(DPV)具有极强的传染性和致命性,所以迫切需要抗病毒药物。我们先前的研究表明DPV感染鸭胚成纤维细胞（DEF）可诱导活性氧（ROS）变化并促进细胞凋亡。在这项研究中,我们测试了羰基氰化物间氯苯基腙（CCCP）的抗病毒作用，一种常见的线粒体自噬诱导剂。我们的结果证明了CCCP的剂量依赖性抗DPV效应，CCCP治疗阻断了感染后DPV的细胞间传播，我们还证明CCCP具有高达48hpi的抗病毒作用。CCCP的加入逆转了DPV诱导的ROS变化，CCCP可抑制病毒诱导的细胞凋亡;同时,CCCP可以影响线粒体融合并激活线粒体自噬以抑制DPV。总之,CCCP可以是针对DPV的有效抗病毒候选物。
