■Pebrine,由微孢子虫引起,是一种毁灭性的疾病,对蚕桑产业造成严重的经济损失。关于开发治疗和诊断方案以管理蚕中的泥水的研究非常有限。微孢子虫的甲硫氨酸氨肽酶2型(MetAP2)是其生存的必需基因,已被用作几种微孢子虫中烟曲霉素及其类似物等药物的细胞靶标。,包括蜜蜂的Nosema。
■在本研究中,使用分子和生物信息学工具,我们对中国广东省分离的家蚕微孢子虫的MetAP2进行了深入的表征和系统发育分析。
■家蚕微虫(广东分离株)的MetAP2基因序列全长为1278个碱基对(bp),包括1077bp的开放阅读框,共编码358个氨基酸。生物信息学分析预测了典型的α-螺旋结构元素的存在,以及缺乏跨膜结构域和信号肽。此外,还预测了稳定蛋白质的其他特征。建立了基于同源性的Bombycis(广东分离株)MetAP2的3D模型,具有较高的准确性和可靠性。表达并纯化MetAP2蛋白。发现MetAP2蛋白的观察分子量为~43-45kDa。系统发育分析表明,家蚕Nosema(广东分离株)的MetAP2基因和氨基酸序列与Nosemaspp具有密切的进化关系。野生蚕,但它与微孢子虫不同。其他昆虫,曲霉属。,酿酒酵母,包括人类在内的高等动物。这些分析表明,MetAP2的保护和进化关系与物种关系密切相关。
■本研究提供了坚实的基础信息,有助于优化和开发诊断和治疗方案,以管理中国蚕桑产业中的家蚕细虫感染威胁。
UNASSIGNED: Pebrine, caused by microsporidium Nosema bombycis, is a devastating disease that causes serious economic damages to the sericulture industry. Studies on development of therapeutic and diagnostic options for managing pebrine in silkworms are very limited. Methionine aminopeptidase type 2 (MetAP2) of microsporidia is an essential gene for their survival and has been exploited as the cellular target of drugs such as fumagillin and its analogues in several microsporidia spp., including Nosema of honeybees.
UNASSIGNED: In the present study, using molecular and bioinformatics tools, we performed in-depth characterization and phylogenetic analyses of MetAP2 of Nosema bombycis isolated from Guangdong province of
China.
UNASSIGNED: The full length of MetAP2 gene sequence of Nosema bombycis (Guangdong isolate) was found to be 1278 base pairs (bp), including an open reading frame of 1,077 bp, encoding a total of 358 amino acids. The bioinformatics analyses predicted the presence of typical alpha-helix structural elements, and absence of transmembrane domains and signal peptides. Additionally, other characteristics of a stable protein were also predicted. The homology-based 3D models of MetAP2 of Nosema bombycis (Guangdong isolate) with high accuracy and reliability were developed. The MetAP2 protein was expressed and purified. The observed molecular weight of MetAP2 protein was found to be ~43-45 kDa. The phylogenetic analyses showed that MetAP2 gene and amino acids sequences of Nosema bombycis (Guangdong isolate) shared a close evolutionary relationship with Nosema spp. of wild silkworms, but it was divergent from microsporidian spp. of other insects, Aspergillus spp., Saccharomyces cerevisiae, and higher animals including humans. These analyses indicated that the conservation and evolutionary relationships of MetAP2 are closely linked to the species relationships.
UNASSIGNED: This study provides solid foundational information that could be helpful in optimization and development of diagnostic and treatment options for managing the threat of Nosema bombycis infection in sericulture industry of
China.