苹果戒指腐烂,苹果贮藏期间最常见的采后病害之一,是由葡萄孢菌引起的。目前,疾病管理主要取决于化学杀菌剂的应用。在这里,我们证明了从中国韭菜(Alliumtuberosum)根中分离出的内生菌芽孢杆菌QNF2极大地抑制了B.dophidea菌丝体的生长,在PDA和PDB培养基中最高抑制为73.56%和99.5%,分别进行体外对抗实验。在体内实验中,龙舌兰芽孢杆菌QNF2对接种龙舌兰果盘并浸入龙舌兰培养物中的采后苹果果实的环腐病表现出88.52%和100%的控制效力。分别。此外,龙舌兰芽孢杆菌QNF2挥发性有机化合物(VOCs)也对采后苹果果实的B.dophidea菌丝生长和环腐病表现出明显的抑制作用。此外,龙舌兰芽孢杆菌QNF2严重破坏了龙舌兰芽孢杆菌的菌丝形态。最后,B.telquilensisQNF2显著抑制6个致病相关基因的表达,比如ADH,aldh,aldh3Galm,pdc1,pdc2,参与多希氏芽孢杆菌的糖酵解/糖异生。研究结果证明,龙须菜QNF2是防治采后苹果果实苹果环腐病的有希望的替代方法。
Apple ring rot, one of the most common apple postharvest diseases during storage, is caused by Botryosphaeria dothidea. Presently, the disease management is primarily dependent on chemical fungicide application. Here we demonstrated an endophyte bacterium Bacillus tequilensis QNF2, isolated from Chinese leek (Allium tuberosum) roots considerably suppressed B. dothidea mycelial growth, with the highest suppression of 73.56 % and 99.5 % in the PDA and PDB medium, respectively in vitro confront experiments. In in vivo experiments, B. tequilensis QNF2 exhibited a control efficacy of 88.52 % and 100 % on ring rot disease on postharvest apple fruits inoculated with B. dothidea disc and dipped into B. dothidea culture, respectively. In addition, B. tequilensis QNF2 volatile organic compounds (VOCs) also manifested markedly inhibition against B. dothidea mycelial growth and the ring rot on postharvest apple fruits. Moreover, B. tequilensis QNF2 severely damaged the mycelial morphology of B. dothidea. Finally, B. tequilensis QNF2 significantly repressed the expression of six pathogenicity-related genes, such as adh, aldh, aldh3, galm, pdc1, pdc2, involved in glycolysis/gluconeogenesis of B. dothidea. The findings of the study proved that B. tequilensis QNF2 was a promising alternative for controlling apple ring rot of postharvest apple fruit.