本研究旨在观察黄精超微粉和水提物对大鼠围绝经期综合征的影响,并探讨其作用机制。具体而言,用阴道涂片法筛选出60只雌性(14-15月龄)发情周期紊乱的SD大鼠,随机分为模型对照组,β-雌二醇3-苯甲酸酯基团(0.1mg·kg~(-1)),黄精超微粉(0.25,0.5g·kg~(-1))和黄精水提物(0.25,0.5g·kg~(-1)),另取10只雌性SD大鼠(14~15月龄)作为青年对照组。给药持续6周。围绝经期综合征相关指标如体温,面部和耳朵的微循环血流量,眩晕期,唾液分泌,抓地力,确定骨强度并进行开场试验。免疫系统相关指标如湿重、胸腺和脾脏指数,外周血中T淋巴细胞和亚群的百分比,并测定血液学指标。此外,卵巢相关指标,如动情周期,子宫和卵巢的湿重和指数,卵巢组织形态学,细胞凋亡测定。此外,下丘脑-垂体-卵巢轴(HPO)相关指标,如血清性激素水平,细胞色素P450家族11亚家族A成员1(CYP11A1),细胞色素P450家族19亚家族A成员1(CYP19A1),测定卵巢组织中细胞色素P450家族17亚家族A成员1(P45017A1)。结果表明,黄精的超细粉和水提取物显着降低了体温(肛门,面部和背部温度),耳朵中的微循环血流,和眩晕期,唾液分泌增加,抓地力,骨强度,在野外试验中的总距离和总速度,胸腺和脾脏的湿重和指数,淋巴细胞比率,CD3~+水平,和CD4~+/CD8~+比值,中性粒细胞数量和比例减少,发情周期障碍比率,和卵巢凋亡细胞的数量,增加了湿重和子宫指数,卵巢湿重,抑制素B(INHB)水平,雌二醇(E_2),抗苗勒管激素(AMH),和卵巢CYP11A1和CYP19A1,减少卵泡刺激素(FSH)和黄体生成素(LH)的含量,改善卵巢组织形态。提示黄精超微粉及水提物可改善大鼠自然围绝经期综合征相关症状,增强卵巢功能和免疫功能。机制是它们通过增加雌激素合成来调节HPO轴功能。
This study aims to examine the effect of superfine powder and aqueous extract of Polygonati Rhizomaon on natural perimenopausal syndrome in rats and explore the underlying mechanism. To be specific, a total of 60 female SD rats(14-15 months old) with estrous cycle disorder were screened by the vaginal smear and randomized into model control group, β-estradiol 3-benzoate group(0.1 mg·kg~(-1)), superfine powder of Polygonati Rhizoma group(0.25, 0.5 g·kg~(-1)) and aqueous extract of Polygonati Rhizoma group(0.25, 0.5 g·kg~(-1)), and another 10 female SD rats(14-15 months old) were selected as the youth control group. The administration lasted 6 weeks. Then the perimenopausal syndrome-related indexes such as body temperature, microcirculatory blood flow of face and ear, vertigo period, salivary secretion, grip force, and bone strength were determined and open field test was conducted. The immune system-related indexes such as the wet weight and index of thymus and spleen, percentage of T lymphocytes and subgroups in peripheral blood, and hematological indexes were measured. In addition, the ovary-related indexes such as estrous cycle, the wet weight and index of uterus and ovary, ovarian tissue morphology, and cell apoptosis were determined. Moreover, hypothalamus-pituitary-ovary axis(HPO)-related indexes such as serum sex hormone levels, cytochrome P450 family 11 subfamily A member 1(CYP11A1), cytochrome P450 family 19 subfamily A member 1(CYP19A1), and cytochrome P450 family 17 subfamily A member 1(P450 17A1) in ovarian tissue were measured. The results showed that the superfine powder and aqueous extract of Polygonati Rhizoma significantly decreased body temperature(anal, facial and dorsal temperature), microcirculatory blood flow in the ear, and vertigo period, increased salivary secretion, grip force, bone strength, total distance and total speed in the open field test, wet weight and index of thymus and spleen, lymphocyte ratio, CD3~+ level, and CD4~+/CD8~+ ratio, reduced neutrophil number and ratio, estrous cycle disorder ratio, and number of ovarian apoptotic cells, raised wet weight and index of uterus, wet weight of ovary, levels of inhibin B(INHB), estradiol(E_2), anti-müllerian hormone(AMH), and ovarian CYP11A1 and CYP19A1, decreased follicle-stimulating hormone(FSH) and luteinizing hormone(LH) content, and improved ovarian tissue morphology. It is suggested that the superfine powder and aqueous extract of Polygonati Rhizoma can improve the symptoms associated with natural perimenopausal syndrome in rats and enhance ovarian function and immune function. The mechanism is that they regulate HPO axis function by increasing estrogen synthesis.