泥土氮的输入形式明显影响泥土CO2的排放。作为一种新的氮输入形式,生物炭负载氨氮不仅减少了农田化学氮肥的投入,而且降低了环境治理成本。促进全国化肥零增长意义重大,农田面源污染防治,实现“碳峰”和“碳中和”的国家目标。“通过室内培养实验,不同氮素输入形式对土壤碳排放的影响,酶活性,通过四种处理方法研究了微生物群落:不施肥(CK),单一施用化学氮肥(CF),生物炭联合施用化学氮肥(BF),和生物炭负载氨氮(BN)。结果表明,与CF相比,BF显著增加累积碳排放量(66.24%),而BN没有显著差异。值得注意的是,与BF和BN相比,累积碳排放量显着减少了35.28%。与CF和BF相比,β-葡萄糖苷酶的活性,过氧化物酶,用BN处理的多酚氧化酶显著增加了20.25%和5.20%,分别。与CF相比,BF处理增加了微生物群落的丰富度和群落的多样性,而BN处理降低了微生物群落的丰富度。与BF相比,变形杆菌的相对丰度下降了11.16%,放线菌和类杆菌的相对丰度分别提高了8.12%和5.83%,分别,其中木糖苷酶活性是影响微生物群落结构的最重要的土壤因子。氯氟菌的相对丰度与纤维二糖水解酶活性显著相关,Gemmaatimonadetes的相对丰度与β-葡萄糖苷酶活性显着相关。变形杆菌的相对丰度与累积碳排放量之间存在非常显着的相关性。总结一下,与生物炭与化学氮肥结合使用的生物炭相比,负载氨氮的生物炭显著减少了累积碳排放量,减排效果较好。本研究结果将有利于国家“双碳战略”的落地,“生物天然气产业的健康发展,建设全国绿色种植循环农业体系,实现国家化肥零增长战略。
The form of soil nitrogen input significantly affects soil CO2 emission. As a new form of nitrogen input, biochar-loaded
ammonia nitrogen not only reduces the input of chemical nitrogen fertilizer in farmland but also reduces the cost of environmental treatment. It is of great significance to promote the zero growth of national chemical fertilizer, the prevention and control of farmland non-point source pollution, and the realization of the national goal of \"carbon peak\" and \"carbon neutralization.\" Through an indoor culture experiment, the effects of different nitrogen input forms on soil carbon emission, enzyme activity, and microbial community were studied through four treatments:no fertilization (CK), single application of chemical nitrogen fertilizer (CF), biochar combined application of chemical nitrogen fertilizer (BF), and biochar-loaded
ammonia nitrogen (BN). The results showed that compared with that in CF, BF significantly increased cumulative carbon emissions (66.24 %), whereas BN had no significant difference. It is worth noting that the cumulative carbon emissions were significantly reduced by 35.28 % compared with that in BF and BN. Compared with those in CF and BF, the activities of β-glucosidase, peroxidase, and polyphenol oxidase treated with BN significantly increased by 20.25 % and 5.20 %, respectively. Compared with that in CF, the BF treatment increased microbial community richness and community diversity, whereas the BN treatment decreased microbial community richness. Compared with that in BF, the relative abundance of Proteobacteria decreased by 11.16 %, and the relative abundance of Actinobacteria and Bacteroidota increased by 8.12 % and 5.83 %, respectively, in which xylosidase activity was the most important soil factor affecting microbial community structure. The relative abundance of Chloroflexi was significantly correlated with cellobiose hydrolase activity, and the relative abundance of Gemmatimonadetes was significantly correlated with β-glucosidase activity. There was a very significant correlation between the relative abundance of Proteobacteria and cumulative carbon emissions. To summarize, compared with those under biochar combined with chemical nitrogen fertilizer, biochar loaded with
ammonia nitrogen significantly reduced cumulative carbon emissions, and its emission reduction effect was better. The results of this study will be beneficial to the landing of the national \"double carbon strategy,\" the healthy development of the biological natural gas industry, the construction of the national green cultivation circular agriculture system, and the realization of the national zero growth strategy of chemical fertilizer.