探讨镍纳米粒子(NiNPs)的生殖毒性及其作用机制,秀丽隐杆线虫（C.用/不用1.0、2.5和5.0μgcm-2的NiNP或镍微粒（NiMPs）处理线虫）。生成时间,受精卵数,检测到精子激活和运动性。结果显示,在相同的治疗剂量下，NiNP对秀丽隐杆线虫的生殖毒性高于NiMPs。在秀丽隐杆线虫中观察到的生殖毒性包括育苗大小的减少，受精卵和精子激活，而是增加了世代时间和不完整的精子细胞。NiNPs对秀丽隐杆线虫的生殖毒性可能是由氧化应激引起的。NiNP诱导的秀丽隐杆线虫的生殖毒性与我们先前在大鼠中的结果一致。因此,线虫可作为检测NiNPs暴露早期生殖毒性的替代模型。©2016威利期刊，Inc.EnvironToxicol32:1530-1538,2017。
To investigate the reproductive toxicity and underlying mechanism of nickel nanoparticles (Ni NPs), Caenorhabditis elegans (C. elegans) were treated with/without 1.0, 2.5, and 5.0 μg cm-2 of Ni NPs or nickel microparticles (Ni MPs). Generation time, fertilized egg numbers, spermatide activation and motility were detected. Results indicated, under the same treatment doses, that Ni NPs induced higher reproductive toxicity to C. elegans than Ni MPs. Reproductive toxicities observed in C. elegans included a decrease in brood size, fertilized egg and spermatide activation, but an increase in generation time and out-of-round spermatids. The reproductive toxicity of Ni NPs on C. elegans may be induced by oxidative stress. The reproductive toxicity in C. elegans induced by Ni NPs is consistent with our previous results in the rats. Therefore, C. elegans can be used as an alternative model to detect the early reproductive toxicity of Ni NPs exposure. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1530-1538, 2017.
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