来自黑蝇的抗菌肽(AMP)(Hermetiaillucens,具有广谱抗微生物活性的BSF)是预防植物病原真菌感染的最有前途的绿色替代品;因此,AMP一直是研究的焦点课题。最近,许多研究集中在BSFAMPs对动物病原体的抗菌活性;然而,目前,它们对植物病原真菌的抗真菌活性尚不清楚。在这项研究中,人工合成了基于BSF宏基因组学从34个预测的AMP中选择的7个AMP。当用选定的AMP处理来自半营养性植物病原真菌稻瘟病菌和炭疽病的分生孢子时,三个选定的AMPS-CAD1,CAD5和CAD7显示出高的附着体形成被延长的胚管抑制。此外,抑制的附着层形成的MIC50浓度为40μM,43μM,米曲霉和43μM,而51μM,49μM,并观察到44μM的阿库坦梭菌,分别。包含CAD1,CAD5和CAD7的名为CAD-Con的串联杂种AMP显着增强了抗真菌活性,和MIC50的浓度为15μM和22μM,分别。与野生型相比,当通过CAD1,CAD5,CAD7或CAD-Con使用经过处理的米曲霉或acutatum的分生孢子进行感染测定时,它们的毒力均显着降低。同时,它们的CAD1,CAD5和CAD7的表达水平也可以被激活,并且在用米曲霉或C.acutatum的分生孢子处理BSF幼虫后显著增加。分别。据我们所知,BSFAMPs对植物病原真菌的抗真菌活性,这有助于我们寻找具有抗真菌活性的潜在AMP,为作物生产绿色控制策略的有效性提供证据。
Antimicrobial peptides (
AMPs) from black solider flies (Hermetia illucens, BSF) exhibiting broad-spectrum antimicrobial activity are the most promising green substitutes for preventing the infection of phytopathogenic fungi; therefore,
AMPs have been a focal topic of research. Recently, many studies have focused on the antibacterial activities of BSF
AMPs against animal pathogens; however, currently, their antifungal activities against phytopathogenic fungi remain unclear. In this study, 7
AMPs selected from 34 predicted
AMPs based on BSF metagenomics were artificially synthesized. When conidia from the hemibiotrophic phytopathogenic fungi Magnaporthe oryzae and Colletotrichum acutatum were treated with the selected AMPs, three selected
AMPs-CAD1, CAD5, and CAD7-showed high appressorium formation inhibited by lengthened germ tubes. Additionally, the MIC50 concentrations of the inhibited appressorium formations were 40 μM, 43 μM, and 43 μM for M. oryzae, while 51 μM, 49 μM, and 44 μM were observed for C. acutatum, respectively. A tandem hybrid AMP named CAD-Con comprising CAD1, CAD5, and CAD7 significantly enhanced antifungal activities, and the MIC50 concentrations against M. oryzae and C. acutatum were 15 μM and 22 μM, respectively. In comparison with the wild type, they were both significantly reduced in terms of virulence when infection assays were performed using the treated conidia of M. oryzae or C. acutatum by CAD1, CAD5, CAD7, or CAD-Con. Meanwhile, their expression levels of CAD1, CAD5, and CAD7 could also be activated and significantly increased after the BSF larvae were treated with the conidia of M. oryzae or C. acutatum, respectively. To our knowledge, the antifungal activities of BSF AMPs against plant pathogenic fungi, which help us to seek potential AMPs with antifungal activities, provide proof of the effectiveness of green control strategies for crop production.