Abstract：
Infected bone defect (IBD) is a great challenge in orthopedics, which involves in bone loss and infection. Here, a self-assembling hydrogel scaffold (named AMP-RAD/EXO), integrating antimicrobial peptides(AMPs), RADA16 and BMSCs exosomes with an innovative strategy, is developed and applied in IBD treatment for sustained antimicrobial ability, accelerating osteoblasts proliferation and promoting bone regeneration. AMPs present an excellent ability to inhibit infection, RADA16 is a self-assembling peptide hydrogel for AMPs delivery, and BMSCs exosomes can promote the bone regeneration. The prepared AMP-RAD/EXO exhibited a polyporous 3D structure for imbibition of BMSCs exosomes and migration of osteoblasts. In vitro studies indicate AMP-RAD/EXO can inhibit the growth of Staphylococcus aureus, accelerate the proliferation and migration of BMSCs. More importantly, in vivo results also prove that AMP-RAD/EXO exhibit an excellent effect on IBD treatment. Thus, the prepared AMP-RAD/EXO provides a multifunctional scaffold concept for bone tissue engineering technology.
摘要：
感染性骨缺损(IBD)是骨科的一大挑战,涉及骨质流失和感染。这里,自组装水凝胶支架（名为AMP-RAD/EXO），整合抗菌肽（AMP），RADA16和BMSCs外泌体的创新策略，开发并应用于IBD治疗，以获得持续的抗菌能力，促进成骨细胞增殖，促进骨再生。AMP表现出优异的抑制感染的能力，RADA16是用于AMP递送的自组装肽水凝胶，BMSCs外泌体可以促进骨再生。制备的AMP-RAD/EXO具有多孔3D结构，可用于BMSCs外泌体的吸收和成骨细胞的迁移。体外研究表明AMP-RAD/EXO可以抑制金黄色葡萄球菌的生长,促进BMSCs的增殖和迁移。更重要的是,体内结果还证明AMP-RAD/EXO对IBD治疗表现出优异的效果。因此，制备的AMP-RAD/EXO为骨组织工程技术提供了一种多功能支架的概念。
