ACT

ACT
  • 文章类型: Journal Article
    小细胞肺癌(SCLC)占所有肺癌诊断的约10%至15%,并且由于其高死亡率而代表了紧迫的全球公共卫生挑战。常规治疗SCLC的疗效欠佳,以有限的抗肿瘤作用和频繁复发为特征。在这种情况下,新兴的研究转向免疫疗法与化疗相结合,这是一个快速发展的领域,在改善SCLC患者的临床结局方面显示出希望。通过最初开发用于非小细胞肺癌(NSCLC),这些疗法拓展了SCLC的新治疗途径.目前,一系列新兴热点治疗方法已显示出显著的治疗效果.基于化疗和免疫治疗的融合,以及新的免疫治疗剂的开发,SCLC的治疗已经看到了希望的未来。通过伴随使用化疗,在增强肿瘤免疫微环境方面取得了进展。免疫疗法,和酪氨酸激酶抑制剂(TKI),正如新兴的临床试验数据所证明的那样。此外,涉及免疫疗法的三方方法,靶向治疗,化疗对未来的临床应用来说似乎是吉祥的。克服对免疫治疗后方案的耐药性仍然是一个紧迫的探索领域。最后,双特异性抗体,过继细胞转移(ACT),溶瘤病毒,单一疗法,包括Delta样配体3(DLL3)和具有Ig和ITIM结构域的T细胞免疫受体(TIGIT),以及精准医学,可能是在SCLC中实现治愈结果的前瞻性途径。这篇综述旨在综合现有文献,并强调SCLC治疗的未来方向,承认该领域的持续挑战。此外,新的治疗药物和技术的不断发展使得SCLC治疗的未来越来越乐观.
    Small cell lung cancer (SCLC) constitutes approximately 10% to 15% of all lung cancer diagnoses and represents a pressing global public health challenge due to its high mortality rates. The efficacy of conventional treatments for SCLC is suboptimal, characterized by limited anti-tumoral effects and frequent relapses. In this context, emerging research has pivoted towards immunotherapy combined with chemotherapy, a rapidly advancing field that has shown promise in ameliorating the clinical outcomes of SCLC patients. Through originally developed for non-small cell lung cancer (NSCLC), these therapies have extended new treatment avenues for SCLC. Currently, a nexus of emerging hot-spot treatments has demonstrated significant therapeutic efficacy. Based on the amalgamation of chemotherapy and immunotherapy, and the development of new immunotherapy agents, the treatment of SCLC has seen the hoping future. Progress has been achieved in enhancing the tumor immune microenvironment through the concomitant use of chemotherapy, immunotherapy, and tyrosine kinase inhibitors (TKI), as evinced by emerging clinical trial data. Moreover, a tripartite approach involving immunotherapy, targeted therapy, and chemotherapy appears auspicious for future clinical applications. Overcoming resistance to post-immunotherapy regimens remains an urgent area of exploration. Finally, bispecific antibodies, adoptive cell transfer (ACT), oncolytic virus, monotherapy, including Delta-like ligand 3 (DLL3) and T cell immunoreceptor with Ig and ITIM domains (TIGIT), as well as precision medicine, may present a prospective route towards achieving curative outcomes in SCLC. This review aims to synthesize extant literature and highlight future directions in SCLC treatment, acknowledging the persistent challenges in the field. Furthermore, the continual development of novel therapeutic agents and technologies renders the future of SCLC treatment increasingly optimistic.
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  • 文章类型: Randomized Controlled Trial
    由于COVID-19大流行及其广泛影响,创伤后应激障碍(PTSD)症状的发生率在中国迅速增加。这项研究旨在评估提供接受和承诺疗法(ACT)的移动应用程序在减少PTSD症状方面的有效性和可接受性。221名PTSD症状升高的中国人被随机分配到应用交付的ACT(ACT条件),应用交付的正念(MI状况),或等待列表(WL条件)。在干预前后进行评估。结果显示,与WL组相比,ACT组和MI组的参与者在心理健康结果方面的改善幅度更大。除心理灵活性外,ACT组和MI组之间无显著差异,ACT比MI改善更多(d=-0.37)。与WL相比,ACT组PTSD症状改善更大(d=-0.79),焦虑(d=-0.62),抑郁(d=-0.51),创伤后成长(d=0.46),和心理灵活性(d=0.76)。ACT和MI的辍学率分别为25.76%和39.71%,分别。ACT条件的参与者报告了中等计划满意度。该研究表明,应用提供的ACT可有效减少PTSD症状并改善中国成年人的整体心理健康。
    Due to the COVID-19 pandemic and its extensive effects, the incidence of posttraumatic stress disorder (PTSD) symptoms is rapidly increasing in China. This research aimed to assess the efficacy and acceptability of a mobile application delivering Acceptance and Commitment Therapy (ACT) in reducing PTSD symptoms. 221 Chinese individuals with elevated PTSD symptoms were randomly assigned to app-delivered ACT (ACT condition), app-delivered mindfulness (MI condition), or a waitlist (WL condition). Assessments were performed pre- and post-intervention. The results showed that participants in both the ACT and MI groups had significantly greater improvements across mental health outcomes compared to the WL group. No significant differences were observed between the ACT and MI groups except for psychological flexibility, which improved more in ACT than MI (d = -0.37). Compared to WL, the ACT group showed a greater improvement in PTSD symptoms (d = -0.79), anxiety (d = -0.62), depression (d = -0.51), posttraumatic growth (d = 0.46), and psychological flexibility (d = 0.76). The drop-out rates in the ACT and MI were 25.76% and 39.71%, respectively. Participants in the ACT condition reported medium program satisfaction. The study suggests app-delivered ACT is efficacious in reducing PTSD symptoms and improving overall mental health among Chinese adults.
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  • 文章类型: Journal Article
    交替孢菌的橘子病型产生一种宿主选择性毒素,称为交替孢菌毒素(ACT)。ACT的整体调节和生物合成的分子机制仍然未知。在本研究中,研究了APSES转录因子StuA的功能。StuA被证明是ACT生物合成和真菌毒力所必需的。StuA被发现了,第一次,使用酵母双杂交与pH响应转录调节剂PacC物理相互作用,双分子荧光互补,和GST下拉测定。功能分析显示,StuA和PacC调节与毒素生物合成和毒力有关的基因的表达。通过靶向基因缺失或沉默pacC的stuA突变产生了真菌菌株,该菌株降低了七个毒素生物合成基因(ACCT)的表达和毒素的产生。EMSA分析显示,PacC可以结合编码烯酰辅酶A水合酶的ACTT6启动子和编码ACT途径特异性转录因子的ACTTR启动子。StuA中五个潜在的蛋白激酶A(PKA)磷酸化位点的定点诱变显示,这些位点均不参与ACT的产生,表明StuA在ACT基因表达调控中的功能不依赖于磷酸化。总的来说,我们的结果证实,PacC是与StuA相互作用的ACT生物合成的关键调节因子之一.环境pH值可能在A.alternata发病过程中起决定性作用。我们的结果还揭示了一个以前未被识别的(StuA-PacC)→ACTTR模块,用于交流中ACT的生物合成。在这项研究中的重要性,我们使用链格孢菌作为生物学模型来报道StuA在植物病原真菌中的作用。我们的发现表明,StuA是柑橘链格孢菌毒素(ACT)生物合成和真菌毒力所必需的。此外,StuA与PacC进行物理交互。stuA或pacC的破坏导致七个毒素生物合成基因(ACCT)的表达和毒素产生减少。PacC可以识别并结合ACTT6和ACTTR的启动子区。我们的结果揭示了一个以前未被识别的(StuA-PacC)→ACTTR模块,用于A.alternata中ACT的生物合成,这也为研究其他真菌中的StuA提供了框架。
    OBJECTIVE: In this study, we used Alternaria alternata as a biological model to report the role of StuA in phytopathogenic fungi. Our findings indicated that StuA is required for Alternaria citri toxin (ACT) biosynthesis and fungal virulence. In addition, StuA physically interacts with PacC. Disruption of stuA or pacC led to decreased expression of seven toxin biosynthetic genes (ACCT) and toxin production. PacC could recognize and bind to the promoter regions of ACTT6 and ACTTR. Our results revealed a previously unrecognized (StuA-PacC)→ACTTR module for the biosynthesis of ACT in A. alternata, which also provides a framework for the study of StuA in other fungi.
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  • 文章类型: Journal Article
    新抗原研究的最新进展加速了肿瘤免疫疗法的发展,包括过继细胞疗法(ACTs),癌症疫苗和基于抗体的疗法,特别是实体瘤。随着新一代测序和生物信息学技术的发展,肿瘤特异性抗原(TSA)的快速鉴定和预测已成为可能。与肿瘤相关抗原(TAAs)相比,高免疫原性TSAs为肿瘤个体化免疫治疗提供了新的靶点,可作为预测肿瘤患者生存的前瞻性指标,预后,和免疫检查点阻断反应。这里,总结了新抗原的鉴定和表征以及基于新抗原的TCR-T免疫治疗策略的临床应用,和当前状态,固有的挑战,并讨论了这些策略的临床转化潜力。
    Recent advances in neoantigen research have accelerated the development of tumor immunotherapies, including adoptive cell therapies (ACTs), cancer vaccines and antibody-based therapies, particularly for solid tumors. With the development of next-generation sequencing and bioinformatics technology, the rapid identification and prediction of tumor-specific antigens (TSAs) has become possible. Compared with tumor-associated antigens (TAAs), highly immunogenic TSAs provide new targets for personalized tumor immunotherapy and can be used as prospective indicators for predicting tumor patient survival, prognosis, and immune checkpoint blockade response. Here, the identification and characterization of neoantigens and the clinical application of neoantigen-based TCR-T immunotherapy strategies are summarized, and the current status, inherent challenges, and clinical translational potential of these strategies are discussed.
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  • 文章类型: Randomized Controlled Trial
    目的:评估正念减压(MBSR)对OSA症状的影响,尤其是在主要结果上,白天过度嗜睡(EDS)。
    方法:平行随机对照试验。16例残留EDS(rEDS)的OSA患者被随机分配到标准化的8周MBSR计划或接受和承诺治疗(ACT)的时间匹配计划。这两个项目都是在网上进行的。参与者在基线(前)在线回答问卷,干预后(员额),在干预后3个月(随访),我们不知道他们的组是治疗组还是主动对照组,但不知道分组(部分致盲).三名参与者提前退出。大多数分析是基于其余13名患者。
    结果:MBSR(n=7)和ACT组(n=6)之间的EDS变化在前后之间存在显着差异(Cohen\sd=1.24,CI[0.01,2.42]),并且ACT组患者在术后EDS显着降低(Cohen\sd=1.18和[0.08,2.22])。在Epworth嗜睡量表上平均2.17点的EDS降低达到了该量表上两个点的临床意义的预定条。失眠症状,次要结果,ACT后显著降低(科恩的d=1.43[0.23,2.58])。在MBSR中,参与者和MBSR培训师均认为基于运动的锻炼最有效.
    结论:ACT显示出作为OSA伴rEDS的辅助治疗的潜力,虽然还需要进一步的研究。似乎有希望使用ACT开发具有rEDS的OSA治疗方法,特别是如果它们是针对该患者群体的特定需求而定制的。
    背景:https://www.drks.de;标识符:DRKS00026812。
    Assessing the effects of Mindfulness-Based Stress Reduction (MBSR) on symptoms of OSA, especially on the primary outcome, excessive daytime sleepiness (EDS).
    Parallel randomized controlled trial. 16 OSA patients with residual EDS (rEDS) were randomized and assigned to either a standardized 8-week MBSR program or a time-matched program on Acceptance and Commitment Therapy (ACT). Both programs were conducted online. Participants answered questionnaires online at baseline (pre), post-intervention (post), three months after the intervention (follow-up) and were blinded to whether their group was the treatment or active control group but not to group allocation (partial blinding). Three participants dropped out early. Most analyses are based on the remaining 13 patients.
    There was a significant difference between the MBSR (n = 7) and ACT group (n = 6) in changes of EDS between pre and post (Cohen\'s d = 1.24, CI [0.01, 2.42]) and a significant reduction of EDS for patients in the ACT group at post (Cohen\'s d = 1.18 and [0.08, 2.22]). This EDS reduction averaging 2.17 points on the Epworth Sleepiness Scale reached the prespecified bar for clinical significance of two points on that scale. Insomnia symptoms, a secondary outcome, reduced significantly following ACT (Cohen\'s d = 1.43 [0.23, 2.58]). In MBSR, both participants and the MBSR-trainer judged movement-based exercises to be most efficacious.
    ACT shows potential as adjunctive therapy for OSA with rEDS, although further studies are needed. It seems promising to develop therapeutic approaches for OSA with rEDS using ACT, especially if they are tailored to the needs specific to this patient group.
    https://www.drks.de; Identifier: DRKS00026812.
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  • 文章类型: Journal Article
    认知功能对日常活动至关重要。接受和承诺疗法(ACT)可以通过增强心理灵活性来改善认知功能,但潜在的机制是未知的。本系统评价了ACT对认知功能的有效性。七个研究数据库(PubMed,ProQuest论文和论文,WebofScience,EBSCOhost,CNKI,Scopus,万方)进行了搜索,以收集以英文和中文发表的试验文章。在应用纳入和排除标准后,我们确定了1994-2022年间发表的12项研究,共包括904名参与者.纳入的研究包括组内(N=3)和随机对照试验(RCT,N=9)研究设计。结果测量包括认知量表和行为测量。在12篇文章中,10项研究表明,由于ACT干预,认知功能的某些领域得到了间接改善。我们发现,ACT干预促进心理灵活性的能力是由于其诊断性质。此外,ACT干预的效果在多个认知领域观察到:注意力,主观认知功能,执行功能,和记忆。总之,认知训练师可以考虑练习ACT作为其策略的一部分,以增强个体的心理灵活性和认知功能。
    Cognitive function is essential for daily activities. Acceptance and commitment therapy (ACT) may improve cognitive function by enhancing psychological flexibility, but the underlying mechanism is unknown. This systematic review evaluated the effectiveness of ACT on cognitive function. Seven research databases (PubMed, ProQuest Dissertations and Theses, Web of Science, EBSCOhost, CNKI, Scopus, Wanfang) were searched to collect articles with trials published in English and Chinese. After applying inclusion and exclusion criteria, we identified 12 studies published between 1994 and 2022 that included a combined total of 904 participants. Among the included studies were within-group (N = 3) and randomized controlled trial (RCT, N = 9) study designs. Outcome measures included cognitive scales and behavioral measurements. Of the 12 articles, 10 studies showed improvements in certain domains of cognitive function due indirectly to ACT intervention. We found that the ability of ACT intervention to promote psychological flexibility is due to its transdiagnostic nature. Also, the effects of the ACT intervention were observed in multiple cognitive domains: attention, subjective cognitive function, executive function, and memory. In conclusion, cognitive trainers could consider practicing ACT as part of their strategy to enhance an individual\'s psychological flexibility and cognitive function.
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  • 文章类型: Meta-Analysis
    目的:为了检查ACT对体重(体重指数和体重)的有效性,饮食行为(暴饮暴食,情绪化的饮食,外部饮食和节制饮食),和心理结果(生活质量[QoL],抑郁症,心理灵活性,和体重污名)在超重和肥胖的成年人中。
    方法:七个电子数据库(CINAHL,EMBASE,PubMed,PsycInfoScopus,科克伦图书馆,和WebofScience)从开始到2022年6月17日进行了搜索。使用随机效应模型分析了13项研究和48项独特效应大小。使用加权平均差(WMD)和以Hedges\'g(g)表示的标准化平均差计算汇总效应估计值。异质性使用Q统计进行评估,并使用I2进行解释。
    结果:发现ACT在改善BMI方面有效(k=6,WMD=-.50,95%CI=-.90;-.11,t=-3.25,p=.20,I2=.0%),心理灵活性和体重相关的污名。然而,发现体重无显著变化(k=4,WMD=-0.3395%CI=-1.53;0.87,t=-.88,p=.44,I2=.0%),暴饮暴食(k=4,g=-.34,95%CI=-1.31;0.62,t=-1.13,p=.34,I2=71.1%),情绪化饮食(k=6,g=-.20,95%CI=-0.54;0.15,t=-1.47,p=.20,I2=45.0%),外部进食(k=5,g=-.40,95%CI=0.96;0.16,t=-1.99,p=.12,I2=81.8%),克制进食(k=3,g=0.2295%CI=-0.57;1.01,t=1.19,p=0.36,I2=69.1%),QoL(k=3,g=0.01,95%CI=-1.51;1.52,t=.02,p=.99,I2=90.2%)和抑郁(k=3,g=-.55,95%CI=-1.78;0.67,t=-1.94,p=.19,I2=79.9%)。
    结论:ACT可有效改善体重减轻,但需要更多的研究来确定其有效性以及各种成分影响体重相关结局的潜在机制。
    方法:从现有实证研究的系统评价和荟萃分析中获得的证据。
    OBJECTIVE: To examine the effectiveness of ACT on weight (body mass index and body mass), eating behaviours (binge eating, emotional eating, external eating and restraint eating), and psychological outcomes (quality of life [QoL], depression, psychological flexibility, and weight stigma) among adults with overweight and obesity.
    METHODS: Seven electronic databases (CINAHL, EMBASE, PubMed, PsycInfo Scopus, The Cochrane Library, and Web of Science) were searched from inception through 17 June 2022. 13 studies and 48 unique effect sizes were analyzed using random-effects models. Pooled effect estimates were calculated using weighted mean differences (WMD) and standardized mean differences expressed in Hedges\' g (g). Heterogeneity was assessed using Q-statistics and interpreted using I2.
    RESULTS: ACT was found to be effective in improving weight loss in terms of BMI (k = 6, WMD = - .50, 95% CI = - .90; - .11, t = - 3.25, p = .20, I2 = .0%), psychological flexibility and weight-related stigma. However, non-significant changes were found for body mass (k = 4, WMD = - 0.33 95% CI = - 1.53; 0.87, t = - .88, p = .44, I2 = .0%), binge eating (k = 4, g = - .34, 95% CI = - 1.31; 0.62, t = - 1.13, p = .34, I2 = 71.1%), emotional eating (k = 6, g = - .20, 95% CI = - 0.54; 0.15, t = - 1.47, p = .20, I2 = 45.0%), external eating (k = 5, g = - .40, 95% CI = 0.96; 0.16, t = - 1.99, p = .12, I2 = 81.8%), restraint eating (k = 3, g = .22 95% CI = - 0.57; 1.01, t = 1.19, p = .36, I2 = 69.1%), QoL (k = 3, g = .01, 95% CI = - 1.51; 1.52, t = .02, p = .99, I2 = 90.2%) and depression (k = 3, g = - .55, 95% CI = - 1.78; 0.67, t = - 1.94, p = .19, I2 = 79.9%).
    CONCLUSIONS: ACT could be effective in improving weight loss but more studies are needed to ascertain its effectiveness and the underlying mechanism by which the various components influence weight-related outcomes.
    METHODS: Evidence obtained from a systematic review and meta-analysis of existing empirical studies.
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  • 文章类型: Journal Article
    未经批准:由于COVID-19大流行及其深远影响,在中国,创伤后应激障碍(PTSD)症状的患病率显着增加。然而,在大流行期间,获得可靠和有效的心理治疗的机会仍然有限。移动技术的广泛采用可能提供解决这一差距的新方法。在这项研究中,我们将开发由移动应用程序提供的基于接受和承诺疗法(ACT)的干预措施,并测试其可用性,功效,及其缓解PTSD症状的作用机制。
    未经评估:根据临床医生管理的创伤后应激障碍量表(CAPS-5),共有147名中国参与者被随机分配到干预组(应用交付的ACT),一个积极的比较组(应用交付的正念),或等待列表组。干预组或比较组中的参与者将使用他们各自的应用程序一个月。在线自我报告问卷将用于评估PTSD症状的主要结局和抑郁症的次要结局。焦虑的症状,创伤后的成长。要测试的潜在中介变量是心理灵活性及其组成部分。这些评估将在基线进行,在治疗期间的五次,在治疗结束时,在1个月和3个月的随访中。
    未经授权:据我们所知,这项研究是第一个调查可用性的随机对照试验,功效,以及应用交付的创伤后应激障碍ACT干预机制。此外,这项研究将评估治疗在降低辍学率方面的效果,探索有效的治疗成分,并研究症状变化的机制,这对于提高PTSD干预措施的有效性和可用性将是有价值的。试用注册:ChiCTR2200058408。
    UNASSIGNED: As a result of the COVID-19 pandemic and its far-reaching impact, the prevalence of posttraumatic stress disorder (PTSD) symptoms is increasing significantly in China. Yet access to reliable and effective psychological treatment is still limited during the pandemic. The widespread adoption of mobile technologies may provide a new way to address this gap. In this research we will develop an Acceptance and Commitment Therapy (ACT) based intervention delivered by mobile application and will test its usability, efficacy, and mechanism of its effects in relieving PTSD symptoms.
    UNASSIGNED: A total of 147 Chinese participants with a diagnosis of PTSD according to the Clinician Administered PTSD Scale (CAPS-5) will be randomly assigned to an intervention group (app-delivered ACT), an active comparison group (app-delivered mindfulness), or a waitlist group. Participants in the intervention group or comparison group will use their respective apps for one month. Online self-report questionnaires will be used to assess the primary outcome of PTSD symptoms and the secondary outcomes symptoms of depression, symptoms of anxiety, and posttraumatic growth. The potential mediating variable to be tested is psychological flexibility and its components. These assessments will be conducted at baseline, at five times during treatment, at the end of treatment, and at 1- and 3-month follow-ups.
    UNASSIGNED: As far as we know, this study is the first randomized controlled trial to investigate the usability, efficacy, and mechanism of an app-delivered ACT intervention for PTSD. Furthermore, the research will assess the effect of treatment in reducing dropout rates, explore effective therapeutic components, and investigate mechanisms of symptom change, which will be valuable in improving the efficacy and usability of PTSD interventions.Trial registration: ChiCTR2200058408.
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  • 文章类型: Journal Article
    血液恶性肿瘤占全球癌症的很大比例。血液系统恶性肿瘤的高发病率和死亡率造成了沉重的社会负担。异基因造血干细胞移植广泛用于血液系统恶性肿瘤的治疗。然而,异基因造血干细胞移植后移植物抗宿主病和复发不可避免.一种新兴的治疗方法,过继细胞疗法,已有效用于血液系统恶性肿瘤的治疗。T细胞,自然杀伤(NK)细胞和肿瘤浸润淋巴细胞(TIL)在治疗应用中都具有巨大的潜力,嵌合抗原受体T(CAR-T)细胞疗法尤其具有潜力,但细胞因子释放综合征和脱靶效应是常见的。迫切需要有效的抗癌措施。近年来,已发现双阴性T细胞(CD3+CD4-CD8-)在预防同种异体/异种移植排斥反应和抑制移植物抗宿主病中具有巨大潜力.它们还具有以MHC不受限制的方式杀死来源于血液恶性肿瘤的各种细胞系的实质能力。此外,健康供体扩增的双阴性T细胞在良好生产规范(GMP)条件下冷冻保存后保留其抗肿瘤能力和抑制移植物抗宿主病的能力,这表明双阴性T细胞可能能够用作现成的产品。在这次审查中,我们揭示了双阴性T细胞治疗血液系统恶性肿瘤的潜在治疗能力.我们希望利用这些细胞作为血液恶性肿瘤的新疗法。
    Haematologic malignancies account for a large proportion of cancers worldwide. The high occurrence and mortality of haematologic malignancies create a heavy social burden. Allogeneic haematopoietic stem cell transplantation is widely used in the treatment of haematologic malignancies. However, graft-versus-host disease and relapse after allogeneic haematopoietic stem cell transplantation are inevitable. An emerging treatment method, adoptive cellular therapy, has been effectively used in the treatment of haematologic malignancies. T cells, natural killer (NK) cells and tumour-infiltrating lymphocytes (TILs) all have great potential in therapeutic applications, and chimeric antigen receptor T (CAR-T) cell therapy especially has potential, but cytokine release syndrome and off-target effects are common. Efficient anticancer measures are urgently needed. In recent years, double-negative T cells (CD3+CD4-CD8-) have been found to have great potential in preventing allograft/xenograft rejection and inhibiting graft-versus-host disease. They also have substantial ability to kill various cell lines derived from haematologic malignancies in an MHC-unrestricted manner. In addition, healthy donor expanded double-negative T cells retain their antitumour abilities and ability to inhibit graft-versus-host disease after cryopreservation under good manufacturing practice (GMP) conditions, indicating that double-negative T cells may be able to be used as an off-the-shelf product. In this review, we shed light on the potential therapeutic ability of double-negative T cells in treating haematologic malignancies. We hope to exploit these cells as a novel therapy for haematologic malignancies.
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  • 文章类型: Journal Article
    柳属雌雄异株植物。研究雄性和雌性花序分化和发育的分子调控机制对于分析柳树的性别分化和单性花发育的潜在机制是必要的。然而,目前,在柳树花序发育过程中没有适合稳定表达的内参基因。在这项研究中,以柳柳为研究材料,9个候选内参基因(α-TUB1,α-TUB2,ACT,H2A,DnaJ,CDC2、GAPDH、选择TIP41,β-TUB),和qRT-PCR技术用于检测每个候选参考基因在不同发育阶段的雌花和雄花中的表达,并使用五种算法(geNorm,Normfinder,DeltaCt,BestKeeper,和RefFinder)来综合评价候选参考基因的稳定性。结果表明,ACT和DnaJ在所有样品中均稳定表达,可作为内参基因。此外,通过编码花特异性转录因子的CFS基因在不同样品中的表达模式分析,进一步验证了筛选结果的可靠性.本研究筛选出的稳定内参基因为今后研究苏木雄花和雌花发育相关功能基因的表达分析提供了依据。
    Salix is a dioecious plant. Research on the molecular regulation mechanism of male and female inflorescence differentiation and development is necessary to analyze sex differentiation in the willow and the underlying mechanisms of unisexual flower development. However, at present, there are no reference genes suitable for stable expression in the process of willow inflorescence development. In this study, Salix suchowensis was used as the research material, nine candidate reference genes (α-TUB1, α-TUB2, ACT, H2A, DnaJ, CDC2, GAPDH, TIP41, β-TUB) were selected, and qRT-PCR technology was used to detect the expression of each candidate reference gene in female and male flowers at different developmental stages and using five algorithms (geNorm, Normfinder, Delta Ct, BestKeeper, and RefFinder) to comprehensively evaluate the stability of candidate reference genes. The results showed that ACT and DnaJ were stably expressed in all samples and could be used as reference genes. In addition, the reliability of the screening results was further verified via an expression pattern analysis of the CFS gene that encodes flower specific transcription factor in different samples. The stable reference genes selected in this study provide the basis for future research on the expression analysis of functional genes related to the development of male and female flowers of S. suchowensis.
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