背景:本研究的目的是评估新型生物陶瓷纳米关节水泥的效果,BioAggregate(InnovativeBioceramix,温哥华,BC,加拿大),在附着力上,迁移,和人牙髓细胞(HDPC)的附着,并将其性能与ProRoot矿物三氧化物聚集体(MTA)的性能进行比较(Dentsply,塔尔萨,OK).
方法:用各种稀释度的BioAggregate和MTA提取物处理原代培养的HDPC,以使用细胞计数试剂盒-8(Dojindo,熊本,日本)。使用I型胶原包被的板进行细胞粘附测定。使用体外划痕伤口愈合模型来确定细胞迁移。通过双重免疫荧光标记对黏珠蛋白和纤维肌动蛋白进一步检查了粘着斑的形成和细胞骨架组织。为了评估细胞附着,将HDPC直接接种到材料表面上并通过扫描电子显微镜观察。
结果:暴露于BioAggregate提取物的HDPC在24和48小时的所有提取物浓度下显示出最高的活力,而与对照相比,暴露于原始MTA提取物的细胞在72小时表现出抑制的活力。用BioAggregate提取物处理以浓度依赖性方式增强细胞粘附和HDPC的迁移,优于MTA提取物的诱导效果。免疫荧光染色表明BioAggregate和MTA均优化了粘着斑形成和应力纤维组装。此外,扫描电子显微镜分析显示,附着在BioAggregate上的HDPC比MTA上的细胞更扁平,并表现出更好的铺展。
结论:BioAggregate能够促进细胞粘附,迁移,和HDPC的附件,表明其优异的细胞相容性。因此,BioAggregate似乎是MTA用于纸浆封盖的可能替代品。
BACKGROUND: The aim of the present
study was to evaluate the effects of a novel bioceramic nanoparticular cement, BioAggregate (Innovative Bioceramix, Vancouver, BC, Canada), on the adhesion, migration, and attachment of human dental pulp cells (HDPCs) and to compare its performance with that of ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK).
METHODS: Primary cultured HDPCs were treated with various dilutions of BioAggregate and MTA extracts to assess the cell viability using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). Cell adhesion assay was performed using type I collagen-coated plates. An in vitro scratch wound healing model was used to determine cell migration. Focal adhesion formation and cytoskeleton organization were further examined by double immunofluorescence labeling for
vinculin and fibrous actin. To assess cell attachment, HDPCs were directly seeded onto the material surfaces and observed by scanning electron microscopy.
RESULTS: HDPCs exposed to BioAggregate extracts showed the highest viabilities at all extract concentrations at 24 and 48 hours, whereas cells exposed to original MTA extracts displayed suppressed viabilities at 72 hours compared with the control. Treatment with BioAggregate extracts enhanced cellular adhesion and migration of HDPCs in a concentration-dependent manner, which was superior to the effects induced by MTA extracts. Immunofluorescence staining indicated that both BioAggregate and MTA optimized focal adhesion formation and stress fiber assembly. Furthermore, scanning electron microscopic analysis revealed that HDPCs attached onto BioAggregate were more flattened and exhibited better spreading than cells on MTA.
CONCLUSIONS: BioAggregate is able to promote cellular adhesion, migration, and attachment of HDPCs, indicating its excellent cytocompatibility. Therefore, BioAggregate appears to be a possible alternative to MTA for pulp capping.