背景:Persicariacapatata(Buch。-火腿.exD.Don)H.Gross(P.capitata,PCB),中国苗族的传统毒品,是用于治疗糖尿病肾病(DN)的潜在传统药物。
目的:本研究的目的是研究P.capitata的功能,阐明其对DN的保护机制。
方法:我们在贵州小型猪中注射链脲佐菌素,和P。capatata被添加到猪的饮食中,以治疗DN。在第16周,所有的动物都被宰杀了,样本被收集,并测量了相对DN指数。16SrRNA测序,宏基因组学,代谢组学,RNA测序,利用蛋白质组学技术探讨了大头菌对DN的保护机制。
结果:饮食中补充白头假单胞菌显著降低了疾病的程度,不仅在相对疾病指数方面,而且在苏木精-伊红染色的组织中。多项分析表明,两种微生物(巴氏梭菌和大肠杆菌),五种代谢物(油酸,亚油酸,4-苯基丁酸,18-β-甘草次酸,和麦角甾醇过氧化物),四种蛋白质(ENTPD5,EPHX1,ARVCF和TREH),四个重要的mRNA(编码ENTPD5、EPHX1、ARVCF、和TREH),六个lncRNAs(TCONS_00024194,TCONS_00085825,TCONS_00006937,TCONS_00070981,TCONS_00074099,andTCONS_00097913),两种circRNAs(novel_circ_0001514和novel_circ_0017507)均参与了P.capitata对DN的保护机制。
结论:我们的研究结果为P的研究和应用提供了多维的理论支持。
BACKGROUND: Persicaria capitata (Buch.-Ham. ex D.Don) H.Gross (P. capitata, PCB), a traditional drug of the Miao people in China, is potential traditional drug used for the treatment of diabetic nephropathy (DN).
OBJECTIVE: The purpose of this
study is to investigate the function of P. capitata and clarify its protective mechanism against DN.
METHODS: We induced DN in the Guizhou miniature pig with injections of
streptozotocin, and P. capitata was added to the pigs\' diet to treat DN. In week 16, all the animals were slaughtered, samples were collected, and the relative DN indices were measured. 16S rRNA sequencing, metagenomics, metabolomics, RNA sequencing, and proteomics were used to explore the protective mechanism of P. capitata against DN.
RESULTS: Dietary supplementation with P. capitata significantly reduced the extent of the disease, not only in term of the relative disease indices but also in hematoxylin-eosin-stained tissues. A multiomic analysis showed that two microbes (Clostridium baratii and Escherichia coli), five metabolites (oleic acid, linoleic acid, 4-phenylbutyric acid, 18-β-glycyrrhetinic acid, and ergosterol peroxide), four proteins (ENTPD5, EPHX1, ARVCF and TREH), four important mRNAs (encoding ENTPD5, EPHX1, ARVCF, and TREH), six lncRNAs (TCONS_00024194, TCONS_00085825, TCONS_00006937, TCONS_00070981, TCONS_00074099, and TCONS_00097913), and two circRNAs (novel_circ_0001514 and novel_circ_0017507) are all involved in the protective mechanism of P. capitata against DN.
CONCLUSIONS: Our results provide multidimensional theoretical support for the
study and application of P. capitata.