PDF(Pubmed)
Abstract：
UNASSIGNED: To assess and compare the therapeutic effect of bone marrow mesenchymal stem cells (BM-MSCs) versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin (β-catenin) expression in experimentally induced type I diabetes in albino rat.
UNASSIGNED: Twenty-eight male albino rats were equally divided as follows; Group I: was composed of rats which received no drug. The remaining rats were administrated a single streptozotocin (STZ) (40 mg/kg) intra-peritoneal injection. After affirmation of diabetes induction, the rats were divided into: Group II: Diabetic rats were given no treatment. Group III: Diabetic rats received a single BM-MSCs intravenous injection (1x106 cells). Group IV: Diabetic rats were given a daily insulin subcutaneous injection (5 IU/kg). After 28 days, mandibles were processed and stained by Hematoxylin & Eosin (H&E), Masson\'s trichrome and anti-β-catenin antibody. A statistical analysis was performed to measure positive area% of Masson\'s trichrome and β-catenin.
UNASSIGNED: Dento-alveolar complex tissues and cells of Group II showed destructive changes histologically, while Groups III and IV demonstrated improved histological features. Group II presented almost old collagen in all dento-alveolar complex tissues, and nearly negative β-catenin expression. Groups III and IV revealed a newly formed collagen intermingled with very few areas of old collagen, and both groups showed positive β-catenin immunoreactivity. Statistically, Groups III and IV represented the highest mean values of Masson\'s trichrome area% and β-catenin area%, while Group II reported the lowest mean.
UNASSIGNED: Streptozotocin has a destructive effect on the dento-alveolar complex structure and function. BM-MSCs and insulin show regenerative capacity in STZ-affected periodontal tissues, and statistically, they increase collagen formation and β-catenin expression.
UNASSIGNED: Twenty-eight male albino rats were equally divided as follows; Group I: was composed of rats which received no drug. The remaining rats were administrated a single streptozotocin (STZ) (40 mg/kg) intra-peritoneal injection. After affirmation of diabetes induction, the rats were divided into: Group II: Diabetic rats were given no treatment. Group III: Diabetic rats received a single BM-MSCs intravenous injection (1x106 cells). Group IV: Diabetic rats were given a daily insulin subcutaneous injection (5 IU/kg). After 28 days, mandibles were processed and stained by Hematoxylin & Eosin (H&E), Masson\'s trichrome and anti-β-catenin antibody. A statistical analysis was performed to measure positive area% of Masson\'s trichrome and β-catenin.
UNASSIGNED: Dento-alveolar complex tissues and cells of Group II showed destructive changes histologically, while Groups III and IV demonstrated improved histological features. Group II presented almost old collagen in all dento-alveolar complex tissues, and nearly negative β-catenin expression. Groups III and IV revealed a newly formed collagen intermingled with very few areas of old collagen, and both groups showed positive β-catenin immunoreactivity. Statistically, Groups III and IV represented the highest mean values of Masson\'s trichrome area% and β-catenin area%, while Group II reported the lowest mean.
UNASSIGNED: Streptozotocin has a destructive effect on the dento-alveolar complex structure and function. BM-MSCs and insulin show regenerative capacity in STZ-affected periodontal tissues, and statistically, they increase collagen formation and β-catenin expression.
摘要：
■评估和比较骨髓间充质干细胞（BM-MSCs）与胰岛素对实验诱导的I型糖尿病大鼠下颌骨牙槽复合体胶原形成和β-catenin（β-catenin）表达的治疗效果。
■将28只雄性白化病大鼠平分如下:第I组:由不接受药物的大鼠组成。其余大鼠单次腹膜内注射链脲佐菌素(STZ)(40mg/kg)。在确认糖尿病诱导后，将大鼠分为:第II组:糖尿病大鼠不给予治疗。组III:糖尿病大鼠接受单次BM-MSC静脉内注射(1x106个细胞)。IV组:糖尿病大鼠每日皮下注射胰岛素(5IU/kg)。28天后,下颌骨被苏木精和伊红（H＆E）处理和染色，Masson三色和抗β-连环蛋白抗体。进行统计分析以测量Masson三色和β-catenin的阳性面积百分比。
■第二组牙槽骨复合体组织和细胞在组织学上表现出破坏性变化，而III组和IV组表现出改善的组织学特征。第二组在所有的牙腺-肺泡复合体组织中呈现几乎老的胶原蛋白，和接近阴性的β-catenin表达。III组和IV组显示新形成的胶原蛋白与很少的旧胶原蛋白区域混合，两组均显示β-catenin免疫反应性阳性。统计上,第III组和第IV组代表了马森的三色面积%和β-连环蛋白面积%的最高平均值，而第二组报告的平均值最低。
■链脲佐菌素对牙槽复合物的结构和功能具有破坏性作用。BM-MSCs和胰岛素在受STZ影响的牙周组织中显示出再生能力，和统计，它们增加胶原形成和β-catenin表达。
■将28只雄性白化病大鼠平分如下:第I组:由不接受药物的大鼠组成。其余大鼠单次腹膜内注射链脲佐菌素(STZ)(40mg/kg)。在确认糖尿病诱导后，将大鼠分为:第II组:糖尿病大鼠不给予治疗。组III:糖尿病大鼠接受单次BM-MSC静脉内注射(1x106个细胞)。IV组:糖尿病大鼠每日皮下注射胰岛素(5IU/kg)。28天后,下颌骨被苏木精和伊红（H＆E）处理和染色，Masson三色和抗β-连环蛋白抗体。进行统计分析以测量Masson三色和β-catenin的阳性面积百分比。
■第二组牙槽骨复合体组织和细胞在组织学上表现出破坏性变化，而III组和IV组表现出改善的组织学特征。第二组在所有的牙腺-肺泡复合体组织中呈现几乎老的胶原蛋白，和接近阴性的β-catenin表达。III组和IV组显示新形成的胶原蛋白与很少的旧胶原蛋白区域混合，两组均显示β-catenin免疫反应性阳性。统计上,第III组和第IV组代表了马森的三色面积%和β-连环蛋白面积%的最高平均值，而第二组报告的平均值最低。
■链脲佐菌素对牙槽复合物的结构和功能具有破坏性作用。BM-MSCs和胰岛素在受STZ影响的牙周组织中显示出再生能力，和统计，它们增加胶原形成和β-catenin表达。
