纳米颗粒越来越多地用于医疗产品和设备。它们的特性对于此类应用至关重要，由于粒子特性决定了它们与生物系统的相互作用，and,因此,最终产品的性能和安全性。其中最重要的纳米粒子特性和参数是粒子质量分布，composition,总颗粒质量,和数量集中。在这项研究中,我们利用单粒子电感耦合等离子体飞行时间质谱（spICP-TOFMS）表征复杂生物流体中的无机纳米粒子。我们报告了在线微液滴校准，用于碳包覆的碳化铁纳米颗粒（C/Fe3CNP）的无参考纳米材料和基质匹配校准。作为一个案例研究，我们分析了设计用于靶向血液净化的C/Fe3CNP。通过对NP质量分布的分析，我们研究了NP表面修饰对全血中C/Fe3CNP聚集的影响。我们还证明了通过磁增强过滤器从盐水中去除涂覆的C/Fe3CNP的效率。磁性过滤显示可将水中可检测的C/Fe3CNP的质量浓度降低99.99±0.01％。
Nanoparticles are increasingly used in medical products and devices. Their properties are critical for such applications, as particle characteristics determine their interaction with the biological system, and, therefore, the performance and safety of the final product. Among the most important nanoparticle characteristics and parameters are particle mass distribution, composition, total particle mass, and number concentration. In this study, we utilize single-particle inductively coupled plasma time-of-flight mass spectrometry (spICP-TOFMS) for the characterization of inorganic nanoparticles in complex biological fluids. We report online microdroplet calibration for reference-nanomaterial-free and matrix-matched calibration of carbon-coated iron carbide nanoparticles (C/Fe3C NPs). As a
case study, we analyze C/Fe3C NPs designed for targeted blood purification. Through the analysis of NP mass distributions, we study the effect of the NP surface modification on aggregation of C/Fe3C NPs in whole blood. We also demonstrate the efficiency of removal of coated C/Fe3C NP from saline by magnetically enhanced filters. Magnetic filtering is shown to reduce the mass concentration of detectable C/Fe3C NPs by 99.99 ± 0.01% in water.
