生物大分子的构象与其细胞功能密切相关。方便地,Förster共振能量转移(FRET)具有良好的偶极子-偶极子距离依赖性,因此可以使用荧光光谱法测量和监测这些构象的纳米级空间尺寸。出于这个原因,FRET通常与单分子检测结合使用,以研究广泛的构象动态生化过程。为那些还不熟悉这个主题的人写的,这篇综述旨在向生物化学家介绍与单分子FRET相关的方法,特别强调如何将其与生物分子模拟相结合,以研究核酸和蛋白质之间的多种相互作用。在第一部分,我们强调了与这种综合方法相关的几个概念和实践考虑。在第二部分,我们回顾了一些最近的研究工作,其中使用单分子FRET和生物分子模拟的各种组合来研究涉及不同类型核酸的生化系统的结构和动态特性(例如,DNA和RNA)和蛋白质(例如,折叠和无序)。
The conformations of biological macromolecules are intimately related to their cellular functions. Conveniently, the well-characterized dipole-dipole distance-dependence of Förster resonance energy transfer (FRET) makes it possible to measure and monitor the nanoscale spatial dimensions of these conformations using fluorescence spectroscopy. For this reason, FRET is often used in conjunction with single-molecule detection to
study a wide range of conformationally dynamic biochemical processes. Written for those not yet familiar with the subject, this review aims to introduce biochemists to the methodology associated with single-molecule FRET, with a particular emphasis on how it can be combined with biomolecular simulations to
study diverse interactions between nucleic acids and proteins. In the first section, we highlight several conceptual and practical considerations related to this integrative approach. In the second section, we review a few recent research efforts wherein various combinations of single-molecule FRET and biomolecular simulations were used to
study the structural and dynamic properties of biochemical systems involving different types of nucleic acids (e.g., DNA and RNA) and proteins (e.g., folded and disordered).