背景:牙周病对口腔健康构成重大挑战,涉及影响牙齿支撑结构的炎症。Denticola密螺旋体,一种“红色复合体”生物,在牙周发病机制中起着至关重要的作用,在龈下环境中形成生物膜并导致菌群失调。抗菌治疗是治疗牙周病的关键,需要细致入微的了解关键病原体如T.denticola表现出的易感性模式。目的和目的本研究的目的是调查的抗菌药物敏感性和耐药性的特点,牙周疾病中一种突出的细菌,通过检查其对牙周治疗中常用的各种抗菌剂的反应。方法学从诊断患有牙周疾病的个体中精心收集斑块样品,以确保口腔微生物组的多样化表现。所有的样本都经过培养,在厌氧培养下分离出红色复合菌。在厌氧条件下从这些样品中培养Dinticola密螺旋体分离株,和分子技术被用于物种鉴定。选择一组全面的抗微生物剂来评估树突密螺旋体的反应。采用抗菌梯度法进行体外抗菌药物敏感性试验(AST),采用混合方法,结合了磁盘扩散和稀释方法的元素。结果丁替科拉螺旋体对甲硝唑表现出耐药性,一种对厌氧菌有效的常用抗生素,强调其适用性的局限性。然而,这种细菌对四环素很敏感,亚胺培南,头孢哌酮,氯霉素,克林霉素,和莫西沙星,提供多样化的治疗选择。抗微生物梯度条测试提供了详细的最小抑制浓度(MIC)值,有助于对易感性和抗性模式有细微的理解。结论本研究极大地促进了我们对牙周疾病背景下树突状螺旋体抗菌药物敏感性和耐药性的认识。研究结果强调了定制治疗策略的重要性,并有助于在抗菌药物管理方面做出更广泛的努力。与全球对抗抗生素耐药性的举措保持一致。这项研究为更有效和个性化的牙周护理方法奠定了基础。强调与牙周健康和疾病相关的复杂微生物动力学。
Background Periodontal disease poses a significant oral health challenge, involving inflammatory conditions impacting tooth-supporting structures. Treponema denticola, a \"red complex\" organism, plays a crucial role in periodontal pathogenesis, forming biofilms in subgingival environments and contributing to dysbiosis. Antimicrobial therapy is pivotal in managing periodontal disease, requiring a nuanced understanding of susceptibility patterns exhibited by key pathogens like T. denticola. Aims and objectives This
study aims to investigate the antimicrobial susceptibility and
resistance profiles of Treponema denticola, a prominent bacterium in periodontal disease, by examining its responses to various antimicrobial agents commonly used in periodontal therapy. Methodology Plaque samples were meticulously collected from individuals diagnosed with periodontal disease to ensure a diverse representation of the oral microbiome. All the samples were cultured, and red complex bacteria were isolated under anaerobic culture. Treponema denticola isolates were cultured from these samples under anaerobic conditions, and molecular techniques were employed for species identification. A comprehensive panel of antimicrobial agents was selected to assess the response of Treponema denticola. In vitro antimicrobial susceptibility testing (AST) was conducted using the antimicrobial gradient method, employing a hybrid approach combining elements of disk-diffusion and dilution methods. Results Treponema denticola had exhibited
resistance to metronidazole, a commonly used antibiotic effective against anaerobic bacteria, emphasizing limitations in its applicability. However, the bacterium displayed sensitivity to tetracycline, imipenem, cefoperazone, chloramphenicol, clindamycin, and moxifloxacin, offering diverse therapeutic options. The antimicrobial gradient strip test provided detailed minimum inhibitory concentration (MIC) values, contributing to a nuanced understanding of susceptibility and resistance patterns. Conclusion This
study significantly advances our understanding of Treponema denticola\'s antimicrobial susceptibility and
resistance profiles in the context of periodontal disease. The findings underscore the importance of tailored treatment strategies and contribute to broader efforts in antimicrobial stewardship, aligning with global initiatives to combat antibiotic
resistance. This research lays the foundation for more effective and personalized approaches to periodontal care, emphasizing the intricate microbial dynamics associated with periodontal health and disease.