CIN2/3治疗后复发性宫颈上皮内病变（rCIN2/3）发生在5-15％的病例中。rCIN2/3可能是由于CIN2/3的不完全切除，其中相同的HPV类型和变异仍然存在。rCIN2/3也可能在与初始病变相同HPV类型的不同HPV变体的新感染后发生。这项研究调查了来自baselineCIN2/3和rCIN2/3病变的配对HPV16阳性刮片中的HPV16共有变体。
选择基线时CI为N2/3的女性和治疗后6或12个月的rCIN2/3的配对HPV16阳性宫颈刮片进行全基因组扩增和Illumina测序。比较序列并表征核苷酸随时间的变化。
从14个配对样本中，10在baselineCI中具有相同的共有变体N2/3和rCIN2/3。与基线相比，四个配对样品在复发性疾病中显示一到三个核苷酸变异。
治疗后，在配对的HPV16阳性baselineCIN2/3和rCIN2/3病变的刮片中发现了相同或几乎相同的HPV16共有变体，提示当在两个病变中发现相同的HPV类型时，不需要进行HPV变异分析。这些结果证明了baselineCIN2/3的不完全切除或无法清除原始HPV感染。
Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5-15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16
consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions.
Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized.
From 14 paired samples, 10 had identical
consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline.
Identical or nearly identical HPV16
consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.
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