背景:姜黄素因其药理活性而被用于传统医学,包括抗氧化剂,抗菌,抗癌,和抗癌特性。因此,对姜黄素的敏感和选择性监测具有很高的实际应用需求。
结果:在这项研究中,我们描述了基于氮掺杂的MoS2量子点(N-MoS2QDs)的姜黄素荧光法的构建。使用钼酸钠和Cys作为前体通过溶剂热法构建N-MoS2量子点。加上姜黄素,N-MoS2量子点的亮蓝色荧光被内滤波效应(IFE)猝灭。QD发射明亮的蓝色荧光并且可以通过经由IFE添加姜黄素来猝灭。姜黄素检测的动态范围为0.1-10μM,检测限为59nM。将N-MoS2量子点应用于实际样品中的姜黄素测定,具有良好的回收率。此外,N-MoS2量子点表现出相对较低的细胞毒性,可用于生物样品中基于荧光的成像。
结论:我们的研究表明,该传感器对监测水样中的姜黄素具有良好的选择性,人体尿液样本,姜粉样品,芥末样品,和咖喱样品,回收率令人满意。N-MoS2量子点具有较低的细胞毒性,具有良好的生物相容性,可用于体外细胞成像。
BACKGROUND: Curcumin has been used in traditional medicine because of its pharmacological activity, including antioxidant, antibacterial, anticancer, and anticarcinogenic properties. Therefore, sensitive and selective monitoring of curcumin is highly demand for practical application.
RESULTS: In this study, we describe the construction of a fluorescence method for curcumin assay based on nitrogen-doped MoS2 quantum dots (N-MoS2 QDs). The N-MoS2 QDs are constructed by a solvothermal method using sodium molybdate and Cys as precursors. With the addition of curcumin, the bright blue fluorescence of N-MoS2 QDs is quenched by the inner filter effect (IFE). The QDs emitted bright blue fluorescence and could be quenched by the addition of curcumin via IFE. The dynamic range is the range of 0.1-10 μM for curcumin detection, with a detection limit of 59 nM. N-MoS2 QDs were applied for curcumin assay in real samples with good recovery. In addition, the N-MoS2 QDs exhibited relative low cytotoxicity and could be applied for fluorescence-based imaging in biological samples.
CONCLUSIONS: Our study indicates that the sensor possesses good selectivity to monitor curcumin in water samples, human urine samples, ginger powder samples, mustard samples, and curry samples with satisfactory recoveries. The N-MoS2 QDs possess less cytotoxicity with excellent biocompatibility and were applied for in vitro cell imaging.