骨组织表现出自我修复特性;然而,并非所有缺陷都可以在没有手术干预的情况下修复。骨组织工程提供人造支架,可以作为骨骼再生的临时基质。本研究的目的是制造由聚乳酸制成的支架,聚(ε-己内酯),聚(富马酸丙烯酯),和用生物玻璃改性的聚乙二醇,β磷酸三钙(TCP),和/或硅灰石(W)颗粒。使用凝胶流延法制造支架,并用光学和扫描电子显微镜观察。衰减全反射-傅里叶变换红外(ATR-FTIR),差示扫描量热法(DSC),热重分析(TG),润湿性,并进行了降解试验。组合物中无W的TCP含量最高,亲水性最高(水接触角为61.9±6.3°),降解速率最快(28天内质量损失7%),在PBS中孵育后沉淀CaP的中等能力,对L929细胞无细胞毒性。没有TCP的W含量最高导致最高的疏水性(水接触角为83.4±1.7°),热稳定性最低,降解速度较慢(28天内质量损失3%),并且没有引起CaP沉淀。此外,在第1天观察到一些细胞毒性的迹象。具有TCP和W两者的样品在第4天显示中等性质和最佳细胞相容性。有趣的是,在磷酸盐缓冲盐水(PBS)中孵育后,它们被典型的花椰菜样羟基磷灰石沉积物覆盖,这可能是他们出色生物活性的标志。
Bone tissue exhibits self-healing properties; however, not all defects can be repaired without surgical intervention. Bone tissue engineering offers artificial scaffolds, which can act as a temporary matrix for bone regeneration. The aim of this study was to manufacture scaffolds made of poly(lactic acid), poly(ε-caprolactone), poly(propylene fumarate), and poly(ethylene glycol) modified with bioglass, beta tricalcium phosphate (TCP), and/or wollastonite (W) particles. The scaffolds were fabricated using a gel-casting method and observed with optical and scanning electron microscopes. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimetry (TG), wettability, and degradation tests were conducted. The highest content of TCP without W in the composition caused the highest hydrophilicity (water contact angle of 61.9 ± 6.3°), the fastest degradation rate (7% mass loss within 28 days), moderate ability to precipitate CaP after incubation in PBS, and no cytotoxicity for L929 cells. The highest content of W without TCP caused the highest hydrophobicity (water contact angle of 83.4 ± 1.7°), the lowest thermal stability, slower degradation (3% mass loss within 28 days), and did not evoke CaP precipitation. Moreover, some signs of cytotoxicity on day 1 were observed. The samples with both TCP and W showed moderate properties and the best cytocompatibility on day 4. Interestingly, they were covered with typical cauliflower-like hydroxyapatite deposits after incubation in phosphate-buffered saline (PBS), which might be a sign of their excellent bioactivity.