磷酸肌醇在许多生物活性中起着重要的作用,对整体细胞功能至关重要。由于其复杂的化学结构,本地化,和低丰度,当前在磷酸肌醇领域的挑战包括准确测量和鉴定特定的变体,特别是那些有酰基链的。研究人员正在大力开发创新技术和方法来应对这些挑战,并提高我们对磷酸肌醇信号传导对细胞生物学影响的理解。本文综述了近年来磷酸肌醇的研究进展,包括质谱,脂质生物传感器,和使用荧光传感器的实时活性测定。这些方法已被证明有助于全面探索磷酸肌醇的细胞分布和动力学,并阐明了这些脂质在人类健康和各种病理过程中日益重要的意义。包括癌症.为了说明磷酸肌醇信号在疾病中的重要性,这种观点也强调了一个名为磷脂酰肌醇5-磷酸4-激酶(PI5P4Ks)的脂质激酶家族的作用,最近已成为癌症治疗的令人兴奋的治疗靶点。对磷酸肌醇信号转导的持续探索不仅加深了我们对细胞生物学的理解,而且为癌症治疗中的新型干预措施提供了希望。
Phosphoinositides serve as essential players in numerous biological activities and are critical for overall cellular function. Due to their complex chemical structures, localization, and low abundance, current challenges in the
phosphoinositide field include the accurate measurement and identification of specific variants, particularly those with acyl chains. Researchers are intensively developing innovative techniques and approaches to address these challenges and advance our understanding of the impact of
phosphoinositide signaling on cellular biology. This article provides an overview of recent advances in the
study of phosphoinositides, including mass spectrometry, lipid biosensors, and real-time activity assays using fluorometric sensors. These methodologies have proven instrumental for a comprehensive exploration of the cellular distribution and dynamics of phosphoinositides and have shed light on the growing significance of these lipids in human health and various pathological processes, including cancer. To illustrate the importance of
phosphoinositide signaling in disease, this perspective also highlights the role of a family of lipid kinases named phosphatidylinositol 5-phosphate 4-kinases (PI5P4Ks), which have recently emerged as exciting therapeutic targets for cancer treatment. The ongoing exploration of
phosphoinositide signaling not only deepens our understanding of cellular biology but also holds promise for novel interventions in cancer therapy.