化学蛋白质组学和质谱脂质组学的进步为了解脂质激酶活性提供了新的机会,特异性,和全球细胞尺度的监管。这里,我们描述了脂质激酶化学生物学的最新进展,重点是磷酸化二酰基甘油的成员。我们进一步讨论了这些基于质谱的方法如何适应其他脂质激酶成员的研究的未来意义,目的是弥合蛋白质和脂质激酶集中的研究之间的差距。
Advancements in chemical proteomics and mass spectrometry lipidomics are providing new opportunities to understand lipid kinase activity, specificity, and regulation on a global cellular scale. Here, we describe recent developments in chemical biology of lipid kinases with a focus on those members that phosphorylate diacylglycerols. We further discuss future implications of how these mass spectrometry-based approaches can be adapted for studies of additional lipid kinase members with the aim of bridging the gap between protein and lipid kinase-focused investigations.