开发有效的废水处理策略以去除微污染物至关重要。因此,在这项研究中,我们提出了一种基于市售纤维素膜和氧化还原酶(包括漆酶)生产生物催化膜的快速方法,酪氨酸酶,和辣根过氧化物酶.基于傅里叶变换红外光谱证实了有效的酶沉积,而分光光度测量结果表明,所有提出的系统的固定化产率超过80%,然后超过80%的活性恢复,膜漆酶系统的值最高(超过90%)。Further,提高了固定化酶的储存稳定性和可重用性,之后达到75%以上,分别,储存20天,和10个重复的生物催化循环。研究的关键阶段涉及使用生产的膜去除血卟啉,(2,4-二氯苯氧基)乙酸(2,4-D),17α-乙炔基雌二醇,四环素,叔戊醇(麻醉药),和酮洛芬甲酯来自污水处理厂不同地方的实际废水采样。尽管生产的膜显示出混合的去除率,所有分析的化合物至少部分地从废水中除去。获得的数据清楚地表明,然而,废水基质的成分,污染物的类型以及酶的类型强烈影响废水的酶处理效率。
The development of efficient strategies for wastewater treatment to remove micropollutants is of the highest importance. Hence, in this
study, we presented a rapid approach to the production of biocatalytic membranes based on commercially available cellulose membrane and
oxidoreductase enzymes including laccase, tyrosinase, and horseradish peroxidase. Effective enzyme deposition was confirmed based on Fourier transform infrared spectra, whereas results of spectrophotometric measurements showed that immobilization yield for all proposed systems exceeded 80% followed by over 80% activity recovery, with the highest values (over 90%) noticed for the membrane-laccase system. Further, storage stability and reusability of the immobilized enzyme were improved, reaching over 75% after, respectively, 20 days of storage, and 10 repeated biocatalytic cycles. The key stage of the
study concerned the use of produced membranes for the removal of hematoporphyrin, (2,4-dichlorophenoxy)acetic acid (2,4-D), 17α-ethynylestradiol, tetracycline, tert-amyl alcohol (anesthetic drug), and ketoprofen methyl ester from real wastewater sampling at various places in the wastewater treatment plant. Although produced membranes showed mixed removal rates, all of the analyzed compounds were at least partially removed from the wastewater. Obtained data clearly showed, however, that composition of the wastewater matrix, type of pollutants as well as type of enzyme strongly affect the efficiency of enzymatic treatment of wastewater.