目的本研究旨在探讨其表达的相关性,通过免疫组织化学,蛋白质OPN,ABCB5和WNT3A来自从石蜡块获得的解剖病理学材料,幻灯片,或者两者兼而有之,来自骨肉瘤(OS)患者,分析流行病学特征,以及它们的存在和对疾病的演变和进展的影响。方法在初步病例选择后,我们搜索了各自的石蜡块,并仅取了那些有足够肿瘤质量的石蜡块,以允许额外的切片而没有生物材料的完全损失.在BenchMarkULTRA(RocheDiagnosticsCorporation,印第安纳波利斯,IN,美国)仪器。然后,我们分析了ABCB5、WNT3A、和骨桥蛋白(OPN)标记与可变年龄,location,和组织类型(Fisher精确检验/卡方检验)。结果患者平均年龄23岁,男性和女性的比率相同。我们分析了2005年至2017年28例OS患者的40张幻灯片。随访时间为80.0个月,5年生存率为46.7%。大多数转移发生在肺组织(92.9%)。蛋白质ABCB5,OPN,与年龄组相比,WNT3A没有统计学意义,新佐剂,佐剂,或者两者兼而有之,化疗,location,生存,或死亡。骨桥蛋白在所有样品中均为阴性。WNT3A表达发生在早期死亡的患者中。结论在免疫组织化学研究中,ABCB5,OPN,WNT3A没有统计学意义。在分析的参数中,它们似乎不是操作系统的预测或积极因素。
Objective This study aimed to correlate the expression, by immunohistochemistry, of the proteins OPN, ABCB5, and WNT3A from anatomopathological materials obtained from paraffin blocks, slides, or both, from patients with osteosarcoma (OS), analyzing epidemiological characteristics, as well as their presence and influence on the evolution and progression of the disease. Methods After the initial case selection, we searched for the respective paraffin blocks and took only those with sufficient tumor mass to allow additional sections with no complete loss of biological material. The sarcoma area identification in representative paraffin blocks used multisample blocks (tissue microarray [TMA]) created on a BenchMark ULTRA (Roche Diagnostics Corporation, Indianapolis, IN, USA) instrument. Then, we analyzed the association between the expression of ABCB5, WNT3A, and
osteopontin (OPN) markers with the variables age, location, and tissue type (Fisher exact test/Chi-squared test). Results The average age of the patients was 23 years, and the rate of males and females was the same. We analyzed 40 slides from 28 OS patients seen from 2005 to 2017. Their follow-up time was 80.0 months, and the 5-year survival rate was 46.7%. Most metastases occurred in lung tissue (92.9%). Proteins ABCB5, OPN, and WNT3A did not present statistical significance when compared with age group, neo-adjuvant, adjuvant, or both, chemotherapy, location, survival, or death.
Osteopontin was negative in all samples. WNT3A expression occurred in patients who died early. Conclusion In an immunohistochemical study, ABCB5, OPN, and WNT3A did not have statistical significance. In the parameters analyzed, they did not seem to be a predictive or aggressive factor for OS.