背景:研究已经描述了微小RNA(miRNA)在胸腺功能中的作用,同时直接观察重症肌无力(MG)患者胸腺中miRNAs表达的改变;miRNAs成为MG病理生理学的核心成分。然而,因为miRNA分析结果是矛盾的,MG相关miRNAs的鉴定令人生畏。
目的:我们对MG患者外周血和单核细胞miRNA表达谱的研究进行了系统综述。
方法:我们在PubMed中运行了一个数据库搜索,Scopus,和WebofScience于2021年8月17日发布。分析外周血miRNA谱的原始文章(血清,等离子体,与非MG或健康对照(HC)组相比,MG患者的全血)和外周血单核细胞(PBMC)均合格。使用诊断准确性研究质量评估2(QUADAS-2)评估研究质量。
结果:纳入26项研究。研究质量是公平的(中位数得分,5).在至少一项研究中失调的226种不同的miRNA中(范围,1-87),在三项或更多项研究中,10个miRNA显著失调。5个miRNA(50%)显示出相同的失调:miR-106b-3p和miR-21-5p一致上调,和miR-20b,miR-15b,和miR-16持续下调。此外,有五个miRNA大部分被上调,miR-150-5p,miR-146a,miR-30e-5p,和miR-338-3p,或下调,miR-324-3p,跨研究。
结论:这些miRNA有助于不同的途径,重要的是神经细胞凋亡和自噬,炎症,调节性T细胞发育,和T辅助细胞平衡。在用于诊断和治疗目的之前,需要追求这些一致且大部分失调的miRNAs在MG背景下特别使用的分子机制。
BACKGROUND: Studies have described the role of microRNAs (miRNAs) in thymic function, along with directly observing the altered expression of miRNAs in thymuses of myasthenia gravis (MG) patients; so, miRNAs became a core component in the pathophysiology of MG. However, because the miRNA analysis results are contradictory, the identification of MG-related miRNAs is daunting.
OBJECTIVE: We did a systematic
review of studies analyzing the miRNA expression profile of peripheral blood and mononuclear cells for patients with MG.
METHODS: We ran a database search in PubMed, Scopus, and Web of Science on August 17, 2021. Original articles that analyzed miRNA profiles in peripheral blood (serum, plasma, and whole blood) and peripheral blood mononuclear cells (PBMCs) for patients with MG in comparison with a non-MG or healthy control (HC) group were eligible. The quality of studies was assessed using the Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2).
RESULTS: 26 studies were included. The quality of studies was fair (median score, 5). Among 226 different miRNAs that were deregulated in at least one study (range, 1-87), ten miRNAs were significantly deregulated in three or more studies. Five miRNAs (50%) showed the same deregulation: miR-106b-3p and miR-21-5p were consistently upregulated, and miR-20b, miR-15b, and miR-16 were consistently downregulated. Also, there were five miRNAs that were mostly upregulated, miR-150-5p, miR-146a, miR-30e-5p, and miR-338-3p, or downregulated, miR-324-3p, across studies.
CONCLUSIONS: These miRNAs contribute to different pathways, importantly neural apoptosis and autophagy, inflammation, T regulatory cell development, and T helper cell balance. Prior to being used for diagnostic and therapeutic purposes, it is required to pursue molecular mechanisms these consistently and mostly dysregulated miRNAs specifically use in the context of MG.