背景:在过去的十年中,已记录了几例支气管肺滴虫病(BPL)。关于BPL患者中Lophomonas感染的流行病学方面的信息很少。本研究的目的是调查Lophomonasspp的患病率。被转诊到伊朗国家滴虫病登记中心(INRCL)的患者的感染,使用形态学和分子测试。
方法:我们检查了2017年至2019年在Mazandaran医科大学参加INRCL的患者,伊朗北部。通过显微镜和小亚基核糖体RNA(SSUrRNA)PCR方法检查了患者的所有支气管肺泡灌洗液(BALF)和两个鼻腔分泌物。为了确认Lophomonas的种类,对两个阳性样本进行了测序。
结果:在这项研究中,对321个标本(包括319个BALF和2个鼻腔分泌物)进行了显微镜检查。Lophomonasspp.发现45例(14%)(n=44BAL;n=1鼻分泌物)。感染患者的平均年龄为54.9±17.1岁。在新鲜涂片和Papanicolaou染色涂片中均观察到以下形态特征,以鉴定Lophomonasspp。用属特异性PCR技术确认所有显微镜阳性标本。将获得的序列以登录号(MN243135-36)存入Gen银行。我们的两个序列的BLAST分析与唯一可用的序列在Gen银行的泰国菌株的布拉塔罗姆,显示99-100%和98.51%的同一性,分别。
结论:据我们所知,这是在全球范围内进行的第一项基于注册登记的关于滴虫病的研究.根据我们的研究,常规PCR检测是临床样本中确认落虫纲寄生虫的可靠工具.此外,结果证实,至少在我们地区,枯萎病菌正在流行。
BACKGROUND: In the last decade, several cases of bronchopulmonary lophomoniasis (BPL) have been recorded. Little information is available about epidemiological aspects on Lophomonas infection among BPL patients. The present
study was aimed to investigate the prevalence of Lophomonas spp. infection in patients who were referred to the Iranian National Registry Center for Lophomoniasis (INRCL), using morphological and molecular tests.
METHODS: We examined patients enrolled in the INRCL from 2017 to 2019 at the Mazandaran University of Medical Sciences, northern Iran. All bronchoalveolar lavage fluid (BALF) and two nasal discharges of the patients were examined by both microscopic and small-subunit ribosomal RNA (SSU rRNA) PCR methods. To confirm the species of Lophomonas, two positive samples were sequenced.
RESULTS: In this
study, 321 specimens (including 319 BALF and 2 nasal discharges) were microscopically examined. Lophomonas spp. was found in 45(14%) (n = 44 BAL; n = 1 nasal discharge). The mean age of infected patients was 54.9 ± 17.1 years. The following morphological characteristics were observed in both fresh and Papanicolaou-stained smears to identify Lophomonas spp. All microscopically positive specimens were confirmed with genus-specific PCR technique. The obtained sequences were deposited in Gen Bank under the accession numbers (MN243135-36). The BLAST analysis of our two sequences with the only available sequence in the Gen Bank of the Thailand strain of L. blattarum, showed identity of 99-100% and 98.51%, respectively.
CONCLUSIONS: To the best of our knowledge, this is the first registry-based
study regarding lophomoniasis worldwide. According to our
study, the conventional PCR test is an available and reliable tool for confirming the Lophomonas parasite in clinical samples. Moreover, the results confirmed that L. blattarum is circulating at least in our region.