There is an increasing awareness of how damaging pollutants in the marine environment can be, however information on the effects of metal engineered nanoparticles (ENPs) on marine biota is still insufficient, despite an exponential rising in related publications in recent years. In order to provide an integrated insight on the present state of the art on metal ENP-related ecotoxicology studies on marine fauna, this review aimed to: (i) highlight the means of toxicity of metal ENPs in the marine environment, (ii) identify the principal biotic and abiotic factors that may alter metal ENP toxicity, and (iii) analyse and categorize results of these studies, including accumulation, molecular and histological biomarkers, genotoxicity and behavioural changes. Data retrieved from Scopus yielded 134 studies that met pre-established criteria. Most often, the target ENPs were titanium, zinc, copper or silver, and most studies (61.2%) focused on the phylum Mollusca. The degree of toxicity of metal ENPs was often dependent on the concentrations tested, length of exposure and the type of tissue sampled. Effects from simple tissue accumulation to DNA damage or behavioural alterations were identified, even when concentrations below environmentally available levels were used. It is proposed that other phyla besides the traditional Mollusca (and within it Bivalvia) should be used more often in this kind of studies, that exact pathways of toxicity be further explored, and lastly that co-stressors be used in order to best mimic conditions observed in nature. In this review, the current knowledge on engineered metal nanoparticles and their effects on marine fauna was summarized, highlighting present knowledge gaps. Guidelines for future studies focusing on under-developed subjects in ENP toxicology are also briefly provided.

Marine seismic surveys produce high intensity, low-frequency impulsive sounds at regular intervals, with most sound produced between 10 and 300Hz. Offshore seismic surveys have long been considered to be disruptive to fisheries, but there are few ecological studies that target commercially important species, particularly invertebrates. This review aims to summarise scientific studies investigating the impacts of low-frequency sound on marine fish and invertebrates, as well as to critically evaluate how such studies may apply to field populations exposed to seismic operations. We focus on marine seismic surveys due to their associated unique sound properties (i.e. acute, low-frequency, mobile source locations), as well as fish and invertebrates due to the commercial value of many species in these groups. The main challenges of seismic impact research are the translation of laboratory results to field populations over a range of sound exposure scenarios and the lack of sound exposure standardisation which hinders the identification of response thresholds. An integrated multidisciplinary approach to manipulative and in situ studies is the most effective way to establish impact thresholds in the context of realistic exposure levels, but if that is not practical the limitations of each approach must be carefully considered.