■妇科乳房发育是最常见的男性乳房疾病,约占男性乳房病变的85%。关于妇科乳房发育的免疫组织化学特征的信息很少。我们的目的是全面分析大量的妇科乳房发育样本,用于推定的乳腺诊断,预测和预后标志物。
■总共156个样品,进行了组织学检查,组装到组织微阵列上,并对雌激素受体(ERα,ERβ1,ERβ2),孕激素受体(总PR,PRα),雄激素受体(AR),基底和腔细胞角蛋白(CK5/6、CK14、CK8/18)和增殖标志物Ki67。标记阳性的相关截止值是根据现有文献定义的:AR(10%),ERα和PR(Allred评分>3/8),ERβ(10%和20%),细胞角蛋白(10%)和Ki67(10%和20%)。
■来自86名13-75岁患者的108个样本可用于免疫组织化学评估。73.1%的病灶为AR阳性,而ERα为99%,ERβ1和ERβ2为100%。98%的样本总PR阳性,97.1%的样本PRα阳性。69.8%表达CK5/6,57%为CK14阳性。还观察到细胞角蛋白表达的三层模式。Ki67阳性较低,分别使用10%和20%的截止值将其分类为Ki67阳性,分别为17.1%和6.7%。ERα表达与患者年龄之间存在显着负相关(rs=-0.221,p=0.023)。产生了双变量相关性,与以前发表的关于正常女性乳腺组织中免疫组织化学状态的数据进行比较,女性和男性乳腺的增生性和肿瘤性乳腺疾病。
■激素受体,包括雌激素受体α和β亚型以及雄激素受体在妇科乳腺发育的导管内腔内增生上皮中大量表达,支持激素在发病机制和治疗中的作用。ERα,与它们在女性乳腺良性病变中的表达相比,ERβ1和ERβ2在更高比例的细胞中表达,这进一步表征了妇科乳房发育的生物学特征。在妇科乳房发育中,低Ki67增殖指数和混合的细胞角蛋白谱的鉴定将这种良性疾病与男性乳腺癌区分开。因此,在常规诊断工作中,Ki67和细胞角蛋白可以帮助从组织学模拟中进行鉴别诊断。
UNASSIGNED: Gynaecomastia is the commonest male breast condition accounting for approximately 85% of male breast lesions. There is minimal information on the immunohistochemical profile of gynaecomastia. We aimed to comprehensively profile a large series of gynaecomastia samples for putative mammary diagnostic, predictive and prognostic markers.
UNASSIGNED: A total of 156 samples, were histologically reviewed, assembled onto tissue microarrays, and stained for oestrogen receptors (ERα, ERβ1, ERß2), progesterone receptors (total PR, PRα), androgen receptor (AR), basal & luminal cytokeratins (CK5/6, CK14, CK8/18) and the proliferation marker Ki67. Relevant cut offs for marker positivity were defined based on existing literature: AR (10%), ERα and PR (Allred score >3/8), ERß (10% and 20%), cytokeratins (10%) and Ki67 (10% and 20%).
UNASSIGNED: 108 samples from 86 patients aged 13-75 years were available for immunohistochemical assessment. 73.1% of the lesions were AR positive, compared to 99% for ERα and 100% for both ERß1 and ERß2. 98% of samples were positive for total PR and 97.1% for PRα. 69.8% expressed CK5/6 whilst 57% were CK14 positive. A tri-layered pattern of cytokeratin expression was also observed. Ki67 positivity was low with 17.1% and 6.7% classified as Ki67 positive using 10% and 20% cut off values respectively. A significant negative correlation was found between ERα expression and patient age (rs = -0.221, p=0.023). Bivariate correlations were produced, and comparisons made with previously published data regarding the immunohistochemical status in normal female breast tissue, proliferative and neoplastic breast diseases of the female and male breast.
UNASSIGNED: Hormone receptors, including oestrogen receptor α and ß isoforms as well as androgen receptors were abundantly expressed within the intraductal luminal hyperplastic epithelium in gynaecomastia supporting the hormonal role in the pathogenesis and treatment. ERα, ERβ1 and ERβ2 were expressed in a higher proportion of cells compared with their expression in the female breast benign lesions which further characterises gynaecomastia biology. The identification of a low Ki67 proliferative index and the mixed cytokeratin profile in gynaecomastia differentiates this benign condition from male breast cancer. Therefore, Ki67 and cytokeratins can help in the differential diagnosis from histological mimics in the routine diagnostic work up.