丛枝菌根真菌(AMF)是生态系统中广泛分布的土壤真菌,可以与大多数陆地植物的根部形成共生关系(菌根)。植物通过菌根协会向AMF提供碳源,而AMF菌丝可以扩大根系对养分的吸收范围,促进植物对养分的吸收。有许多不同种类的AMF,AMF的不同物种与不同植物之间的共生关系各不相同。入侵植物可以通过根系分泌物丰富具有更好共生能力的AMF物种,促进它们的生长,从而增加它们在入侵植物根中的定殖。同时,入侵植物也会破坏AMF与本地植物之间的共生关系,影响当地的植物群落,这是植物成功入侵的机制之一。AMF在入侵植物和本地植物根中的定殖率间接反映了AMF在入侵植物入侵过程中的作用。在这种方法中,收集的植物根可以直接处理或保存在固定剂中供以后的批处理。通过脱色,酸化,染色,和腐烂的根治疗,菌丝,孢子,并且可以清楚地观察到根系中AMF的丛枝结构。该方法可以在基本实验室中完成对AMF在入侵植物根系中定植率的观察和计算。
Arbuscular mycorrhizal fungi (AMF) are widely distributed soil fungi in ecosystems and can form symbiotic associations (mycorrhizae) with the roots of most terrestrial plants. Plants provide carbon sources to AMF through mycorrhizal associations, while AMF
hyphae can expand the range of nutrient absorption by roots and promote plant nutrient uptake. There are many different species of AMF, and the symbiotic relationships between different species of AMF and different plants vary. Invasive plants can enrich AMF species with better symbiotic capabilities through root exudates, promoting their growth and thereby increasing their colonization in invasive plant roots. At the same time, invasive plants can also disrupt the symbiotic relationship between AMF and native plants, affecting the local plant community, which is one of the mechanisms for successful plant invasion. The colonization rate of AMF in the roots of invasive and native plants indirectly reflects the role of AMF in the process of invasive plant invasion. In this method, collected plant roots can be processed directly or saved in a fixative for later batch processing. Through decolorization, acidification, staining, and destaining treatment of roots, the
hyphae, spores, and arbuscular structures of AMF in the root system can be clearly observed. This method can be completed in a basic laboratory to observe and calculate the colonization rate of AMF in the root systems of invasive plants.