人胚胎干细胞(hESCs)具有独特的生物学特征和属性,使其在生物医学研究的各个领域具有吸引力。随着应用程序的增加,蛋白质组分析的发展需求不断增加。膜蛋白是hESC蛋白的最重要子集之一,因为它们可以用作表面标记。涵盖的领域:这篇综述讨论了hESC常用的表面标记,并提供了对可用的hESC膜蛋白质组报告以及许多其他细胞类型中这些标记的存在的深入分析,尤其是癌细胞.欣赏,膜蛋白的定义存在歧义,我们尝试使用蛋白质数据库和预测工具对已发表的hESCs膜蛋白报告进行荟萃分析,以寻找hESCs中最有信心的质膜蛋白.此外,已经基于可用的转录组分析数据库讨论了质膜蛋白对分化的响应性。专家评论:联合转录组和膜蛋白质组分析强调了其他蛋白质,这些蛋白质最终可能会作为新的细胞表面标记物发挥作用。
Human embryonic stem cells (hESCs) have unique biological features and attributes that make them attractive in various areas of biomedical research. With heightened applications, there is an ever increasing need for advancement of proteome analysis. Membrane proteins are one of the most important subset of hESC proteins as they can be used as surface markers. Areas covered: This review discusses commonly used surface markers of hESCs, and provides in-depth analysis of available hESC membrane proteome
reports and the existence of these markers in many other cell types, especially cancer cells. Appreciating, existing ambiguity in the definition of a membrane protein, we have attempted a meta analysis of the published membrane protein
reports of hESCs by using a combination of protein databases and prediction tools to find the most confident plasma membrane proteins in hESCs. Furthermore, responsiveness of plasma membrane proteins to differentiation has been discussed based on available transcriptome profiling data bank. Expert commentary: Combined transcriptome and membrane proteome analysis highlighted additional proteins that may eventually find utility as new cell surface markers.