肉桂疫霉是世界范围内高度流行的植物病原体,在分布方面排名前十。它会导致皇冠腐烂,溃疡,和许多植物物种的根腐病,显著影响受影响环境中动植物的生物多样性。寄主范围超过5000种,包括一些重要的植物,槲寄生,板栗,和商业上重要的作物,如鳄梨(PerseaAmericana),玉米(玉米),和番茄(Solanumlycopersicum),肉桂疫霉对农业和生态系统构成重大威胁。卵菌的有效传播依赖于其短暂的无性繁殖游动孢子,依靠水流感染宿主根。然而,由于生命周期的复杂性,在实验室中管理这些游动孢子长期以来一直具有挑战性。目前的协议涉及复杂的程序,包括交替的增长周期,干旱,和洪水。不幸的是,这些人工条件通常会导致毒力迅速下降,在培养过程中需要额外的步骤来保持感染性。在我们的研究中,我们试图通过调查游动孢子在各种条件下的存活情况来应对这一挑战.我们的目标是开发一种稳定的游动孢子种群,既易于部署又具有高度传染性。通过在液氮中直接冷冻,我们成功地保存了它们的毒力。这一突破消除了重复培养转移的需要,简化植物接种过程。此外,它可以更全面地研究肉桂疫霉及其与寄主植物的相互作用。
Phytophthora cinnamomi Rands is a highly prevalent phytopathogen worldwide, ranking among the top ten in terms of distribution. It inflicts crown rot, canker, and root rot on numerous plant species, significantly impacting the biodiversity of both flora and fauna within affected environments. With a host range spanning over 5,000 species, including important plants like Quercus suber, Quercus ilex, Castanea sativa, and commercially significant crops such as avocado (Persea americana), maize (Zea mays), and tomato (Solanum lycopersicum), Phytophthora cinnamomi poses a substantial threat to agriculture and ecosystems. The efficient dissemination of the oomycete relies on its short-lived asexually motile zoospores, which depend on water currents to infect host roots. However, managing these zoospores in the laboratory has long been challenging due to the complexity of the life cycle. Current protocols involve intricate procedures, including alternating cycles of growth, drought, and flooding. Unfortunately, these artificial conditions often result in a rapid decline in virulence, necessitating additional steps to maintain infectivity during cultivation. In our research, we sought to address this challenge by investigating zoospore survival under various conditions. Our goal was to develop a stable stock of zoospores that is both easily deployable and highly infective. Through direct freezing in liquid nitrogen, we have successfully preserved their virulence. This breakthrough eliminates the need for repeated culture transfers, simplifying the process of plant inoculation. Moreover, it enables more comprehensive studies of Phytophthora cinnamomi and its interactions with host plants.