目的:1)量化抗牙龈卟啉单胞菌血清抗体浓度与发生类风湿关节炎(RA)的风险之间的关系,和2)量化抗P.牙龈血清抗体浓度和RA特异性自身抗体。评估的其他抗细菌抗体包括抗核梭杆菌和抗中间普氏菌。
方法:从美国国防部血清库获得RA诊断前后的血清样本(n=214例,210个匹配的控件)。使用单独的混合模型,反P升高的时间牙龈,反P.intermedia,和反F在RA病例与对照中比较了相对于RA诊断的核仁抗体浓度。确定血清抗CCP2,ACPA细特异性之间的关联(波形蛋白,histone,和α-烯醇化酶),还有IgA,IgG,使用混合效应线性回归模型,RA诊断前样本中的IgMRF和抗菌抗体。
结果:没有令人信服的证据表明血清抗P的病例对照差异。牙龈,反F核仁,和反P中间观察到。在RA病例中,包括所有诊断前的血清样本,反P.中间介质与抗CCP2,ACPA细特异性靶向波形蛋白,histone,α-烯醇化酶,和IgARF(p<0.001),IgG射频(p=0.049),和IgMRF(p=0.004),而反P牙龈和抗F.核仁没有。
结论:与对照组相比,在RA诊断前,未观察到抗细菌血清抗体浓度的纵向升高。然而,反P.在RA诊断前,中间介质显示与RA自身抗体浓度显著相关,提示该生物在临床可检测的RA进展中的潜在作用。
1) To quantify the association between anti-Porphyromonas gingivalis serum antibody concentrations and the risk of developing rheumatoid arthritis (RA), and 2) to quantify the associations among RA cases between anti-P. gingivalis serum antibody concentrations and RA-specific autoantibodies. Additional anti-bacterial antibodies evaluated included anti-Fusobacterium nucleatum and anti-Prevotella intermedia.
Serum samples were acquired pre- and post- RA diagnosis from the U.S. Department of Defense Serum Repository (n = 214 cases, 210 matched controls). Using separate mixed-models, the timing of elevations of anti-P. gingivalis, anti-P. intermedia, and anti-F. nucleatum antibody concentrations relative to RA diagnosis were compared in RA cases versus controls. Associations were determined between serum anti-CCP2, ACPA fine specificities (vimentin, histone, and alpha-enolase), and IgA, IgG, and IgM RF in pre-RA diagnosis samples and anti-bacterial antibodies using mixed-effects linear regression models.
No compelling evidence of
case-control divergence in serum anti-P. gingivalis, anti-F. nucleatum, and anti-P. intermedia was observed. Among RA cases, including all pre-diagnosis serum samples, anti-P. intermedia was significantly positively associated with anti-CCP2, ACPA fine specificities targeting vimentin, histone, alpha-enolase, and IgA RF (p<0.001), IgG RF (p = 0.049), and IgM RF (p = 0.004), while anti-P. gingivalis and anti-F. nucleatum were not.
No longitudinal elevations of anti-bacterial serum antibody concentrations were observed in RA patients prior to RA diagnosis compared to controls. However, anti-P. intermedia displayed significant associations with RA autoantibody concentrations prior to RA diagnosis, suggesting a potential role of this organism in progression towards clinically-detectable RA.