Biothiols, such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play a vital role in gene expression, maintaining redox homeostasis, reducing damages caused by free radicals/toxins, etc. Likewise, abnormal levels of biothiols can lead to severe diseases, such as Alzheimer\'s disease (AD), neurotoxicity, hair depigmentation, liver/skin damage, etc. To quantify the biothiols in a biological system, numerous low-toxic probes, such as fluorescent quantum dots, emissive organic probes, composited nanomaterials, etc., have been reported with real-time applications. Among these fluorescent probes, carbon-dots (CDs) have become attractive for biothiols quantification because of advantages of easy synthesis, nano-size, crystalline properties, low-toxicity, and real-time applicability. A CDs-based biothiols assay can be achieved by fluorescent \"Turn-On\" and \"Turn-Off\" responses via direct binding, metal complex-mediated detection, composite enhanced interaction, reaction-based reports, and so forth. To date, the availability of a review focused on fluorescent CDs-based biothiols detection with information on recent trends, mechanistic aspects, linear ranges, LODs, and real applications is lacking, which allows us to deliver this comprehensive review. This review delivers valuable information on reported carbon-dots-based biothiols assays, the underlying mechanism, their applications, probe/CDs selection, sensory requirement, merits, limitations, and future scopes.

In recent decades, photoluminescence (PL) material with excellent optical properties has been a hot topic. Graphene oxide (GO) is an excellent candidate for PL material because of its unique optical properties, compared to pure graphene. The existence of an internal band gap in GO can enrich its optical properties significantly. Therefore, GO has been widely applied in many fields such as material science, biomedicine, anti-counterfeiting, and so on. Over the past decade, GO and quantum dots (GOQDs) have attracted the attention of many researchers as luminescence materials, but their luminescence mechanism is still ambiguous, although some theoretical results have been achieved. In addition, GO and GOQDs have fluorescence quenching properties, which can be used in medical imaging and biosensors. In this review, we outline the recent work on the photoluminescence phenomena and quenching process of GO and GOQDs. First, the PL mechanisms of GO are discussed in depth. Second, the fluorescence quenching mechanism and regulation of GO are introduced. Following that, the applications of PL and fluorescence quenching of GO-including biomedicine, electronic devices, material imaging-are addressed. Finally, future development of PL and fluorescence quenching of GO is proposed, and the challenges exploring the optical properties of GO are summarized.

Mercuric (Hg2+) ions released from human activities, natural phenomena, and industrial sources are regarded as the global pollutant of world\'s water. Hg2+ ions contaminated water has several adverse effects on human health and the environment even at low concentrations. Therefore, rapid and cost-effective method is urgently required for the detection of Hg2+ ions in water. Although, the current analytical methods applied for the detection of Hg2+ ions provide low detection limit, they are time consuming, require expensive equipment, and are not suitable for in-situ analysis. Metal nanoclusters (MNCs) consisting of several to ten metal atoms are important transition missing between single atoms and plasmonic metal nanoparticles. In addition, sub-nanometer sized MNCs possess unique electronic structures and the subsequent unusual optical, physical, and chemical properties. Because of these novel properties, MNCs as a promising material have attracted considerable attention for the construction of selective and sensitive sensors to monitor water quality. Hence this review is focused on recent advances on synthesis strategies, and optical and chemical properties of various MNCs including their applications to develop optical assay for Hg2+ ions in aqueous solutions.

Early diagnosis of diseases is of great importance because it increases the chance of a cure and significantly reduces treatment costs. Thus, development of rapid, sensitive, and reliable biosensing techniques is essential for the benefits of human life and health. As such, various nanomaterials have been explored to improve performance of biosensors, among which, carbon dots (CDs) have received enormous attention due to their excellent performance. In this Review, the recent advancements of CD-based biosensors have been carefully summarized. First, biosensors are classified according to their sensing strategies, and the role of CDs in these sensors is elaborated in detail. Next, several typical CD-based biosensors (including CD-only, enzymatic, antigen-antibody, and nucleic acid biosensors) and their applications are fully discussed. Last, advantages, challenges, and perspectives on the future trends of CD-based biosensors are highlighted.

Dissolved oxygen is an important index to evaluate water quality, and its concentration is of great significance in industrial production, environmental monitoring, aquaculture, food production, and other fields. As its change is a continuous dynamic process, the dissolved oxygen concentration needs to be accurately measured in real time. In this paper, the principles, main applications, advantages, and disadvantages of iodometric titration, electrochemical detection, and optical detection, which are commonly used dissolved oxygen detection methods, are systematically analyzed and summarized. The detection mechanisms and materials of electrochemical and optical detection methods are examined and reviewed. Because external environmental factors readily cause interferences in dissolved oxygen detection, the traditional detection methods cannot adequately meet the accuracy, real-time, stability, and other measurement requirements; thus, it is urgent to use intelligent methods to make up for these deficiencies. This paper studies the application of intelligent technology in intelligent signal transfer processing, digital signal processing, and the real-time dynamic adaptive compensation and correction of dissolved oxygen sensors. The combined application of optical detection technology, new fluorescence-sensitive materials, and intelligent technology is the focus of future research on dissolved oxygen sensors.

The specific spectroscopic and redox properties of porphyrins predestine them to fulfill the role of sensors during interacting with different biologically active substances. Monitoring of binding interactions in the systems porphyrin-biologically active compound is a key question not only in the field of physiological functions of living organisms, but also in environmental protection, notably in the light of the rapidly growing drug consumption and concurrently the production of drug effluents. Not always beneficial action of drugs on natural porphyrin systems induces to further studies, with commercially available porphyrins as the model systems. Therefore the binding process between several water-soluble porphyrins and a series of biologically active compounds (e.g. caffeine, guanine, theophylline, theobromine, xanthine, uric acid) has been studied in different aqueous solutions analyzing their absorption and steady-state fluorescence spectra, the porphyrin fluorescence lifetimes and their quantum yields. The magnitude of the binding and fluorescence quenching constants values for particular quenchers decreases in a series: uric acid > guanine > caffeine > theophylline > theobromine > xanthine. In all the systems studied there are characters of static quenching, as a consequence of the π-π-stacked non-covalent and non-fluorescent complexes formation between porphyrins and interacting compounds, accompanied simultaneously by the additional specific binding interactions. The porphyrin fluorescence quenching can be explain by the photoinduced intermolecular electron transfer from aromatic compound to the center of the porphyrin molecule, playing the role of the binding site. Presented results can be valuable for designing of new fluorescent porphyrin chemosensors or monitoring of drug traces in aqueous solutions. The obtained outcomes have also the toxicological and medical importance, providing insight into the interactions of the water-soluble porphyrins with biologically active substances.