本研究的目的是确定恩诺沙星及其代谢物的药代动力学,环丙沙星,在南阳牛一次静脉注射后(IV),和肌内(IM)施用2.5mg/kg体重(BW)的恩诺沙星。在预定时间点收集血液样品。使用高效液相色谱(HPLC)分析方法同时测定血浆中恩诺沙星和环丙沙星的浓度,并进行非房室分析。静脉给药后,恩诺沙星在稳态(VSS)下的平均(±SD)分布体积为1.394±0.349L/kg,终末半衰期(t1/2λz)为3.592±1.205h,全身间隙(Cl)为0.675±0.16L/h/kg。IM管理后,恩诺沙星吸收相对缓慢但完全,平均吸收时间(MAT)为6.051±1.107h,生物利用度为99.225±7.389%。两种给药途径后,在大多数血浆样品中同时检测到两种化合物。表明恩诺沙星有效生物转化为环丙沙星。静脉注射后,环丙沙星的峰浓度(Cmax)为0.315±0.017μg/mL,在0.958±0.102h时观察到。IM注射后,相应的值为0.071±0.006μg/mL和3±1.095h,分别。在IV和IM管理之后,恩诺沙星与环丙沙星的转化率分别为59.2±9.6%和31.2±7.7%,分别。目前的结果表明,恩诺沙星的药代动力学特征良好,以相对缓慢的动力学完全吸收为特征,广泛分布,环丙沙星的高效生物转化,并长期消灭南阳牛。
The objective of this study was to determine the pharmacokinetics of
enrofloxacin and its metabolite, ciprofloxacin, in Nanyang cattle after a single intravenous (IV), and intramuscular (IM) administration of enrofloxacin at 2.5 mg/kg body weight (BW). Blood samples were collected at predetermined time points. Enrofloxacin and ciprofloxacin concentrations in plasma were simultaneously determined using a high-performance liquid chromatography (HPLC) assay method and subjected to a non-compartmental analysis. After IV administration,
enrofloxacin had a mean (±SD) volume of distribution at steady state (VSS) of 1.394 ± 0.349 L/kg, a terminal half-life (t1/2λz) of 3.592 ± 1.205 h, and a total body clearance (Cl) of 0.675 ± 0.16 L/h/kg. After IM administration,
enrofloxacin was absorbed relatively slowly but completely, with a mean absorption time (MAT) of 6.051 ± 1.107 h and a bioavailability of 99.225 ± 7.389%. Both compounds were detected simultaneously in most plasma samples following both routes of administration, indicating efficient biotransformation of enrofloxacin to ciprofloxacin. After IV injection, the peak concentration (Cmax) of ciprofloxacin was 0.315 ± 0.017 μg/mL, observed at 0.958 ± 0.102 h. Following IM injection, the corresponding values were 0.071 ± 0.006 μg/mL and 3 ± 1.095 h, respectively. Following IV and IM administration, the conversion ratio of
enrofloxacin to ciprofloxacin was calculated as 59.2 ± 9.6% and 31.2 ± 7.7%, respectively. The present results demonstrated favorable pharmacokinetic profiles for
enrofloxacin, characterized by complete absorption with relatively slow kinetics, extensive distribution, efficient biotransformation to ciprofloxacin, and prolonged elimination in Nanyang cattle.