牙髓干细胞(DPSC)已被推荐为基于细胞的治疗应用的有希望的候选者,因为它在组织修复/再生和免疫反应调节方面具有很高的潜力。通过天然植物增强子的基因表达改变策略是改善这些细胞的干性特性的可用机会。这项研究的目的是评估藏红花植物的生物活性化合物(藏红花植物的生物活性化合物)对人类DPSCs中免疫调节和组织再生相关生物标志物表达的影响。根据细胞活力测定的结果,施用400μM和较低浓度的藏红花素对DPSCs没有毒性作用;然而,在较高浓度下观察到时间依赖性细胞毒性作用.这项研究,可能是第一次,检测到DPSC中CD200的表面表达,并有轻微的时间依赖性上升趋势,并报道用藏红花素处理可以使这种大分子的表达增加很多倍。此外,它揭示了这种类胡萝卜素显著导致牙本质唾液酸磷蛋白的时间依赖性上调,血管内皮生长因子A,人类白细胞抗原-G5和信号转导和转录激活因子-3信使核糖核酸(mRNA);然而,STAT3的这种显著上调发生,其次是显著的减少。这项研究的结果表明,用藏红花素进行细胞处理可能有效地改善DPSC的干性能力。因此,该研究为更深入地了解藏红花素对DPSCs的生物学效应提供了依据,它可能有助于将来改善基于间充质干细胞的疗法。
Dental pulp stem cells (DPSCs) have been recommended as promising candidate for cell-based therapeutic applications due to high potentials in tissue repair/regeneration and modulation of immune responses. The gene expression change strategy by natural plant enhancers is an available opportunity to improve the stemness properties of these cells. The objective of this research was the evaluation of Crocin effects (saffron plant\'s bioactive compound) on immunoregulation and tissue regeneration-related biomarkers expression in human DPSCs. Based on the results of cell viability assay, application of 400 μM and lower concentrations of Crocin had no toxic effects on DPSCs; however, the time-dependent cytotoxic effects were observed at higher concentrations. This
study, probably for the first time, detected the surface expression of CD200 in DPSCs with a slight time-dependent upward trend and reported that treatment with Crocin could increase expression of this macromolecule up to many times over. Also, it revealed that this carotenoid significantly led to the time-dependent upregulation of dentin sialophosphoprotein, vascular endothelial growth factor A, human leukocyte antigen-G5, and signal transducer and activator of transcription-3 messenger ribonucleic acids (mRNAs); however, this significant upregulation for STAT3 occurred, followed by a remarkable reduction. The results of this
study indicated that cell treatment with Crocin may be effective in improving the stemness capacities of DPSCs. Therefore, the
study provided basis for more insights into the biological effects of Crocin on DPSCs that it may aid in the future improvement of mesenchymal stem cell-based therapies.