藏红花苷是藏红花中天然存在的藏红花苷元,已被证明具有抗氧化剂,抗炎,和抗菌活性。在这个实验中,研究了藏红花酸对高原低氧大鼠重要脏器的保护作用。以栀子为原料,采用碱水解法制备藏红花酸,并对其还原能力和自由基清除能力进行了测试。研究了PC12细胞的体外抗缺氧活力。在几种模型中确定了小鼠的抗缺氧存活时间。通过模拟海拔8000m的低氧环境24h,建立急性低氧损伤大鼠模型,并通过腹腔注射10、20和40mg/kg的剂量评价了番红花素的抗缺氧作用。测定了脑和肺的含水量,和大脑中的病理切片,肺,心,肝脏,HE染色观察肾脏。氧化应激水平(SOD,CAT,H2O2,GSH,GSH-Px,MDA)和炎症因子(IL-1β,IL-6,TNF-α,VEGF)在大鼠大脑中,肺,心,肝脏,用ELISA法检测肾组织。结果表明,在低氧条件下,藏红花酸具有较强的还原能力和清除自由基的能力,能提高PC12细胞的活性。腹膜内注射藏红花素后,小鼠的存活时间延长,和病理损伤,氧化应激,大鼠组织的炎症得到改善。藏红花酸对高原低氧大鼠重要脏器的保护作用可能与降低氧化应激、抑制炎症反应有关。
Crocetin is an aglycone of crocin naturally occurring in saffron and has been proved to have antioxidant, anti-inflammatory, and antibacterial activities. In this experiment, the protective effect of
crocetin on vital organs in high-altitude hypoxia rats was studied.
Crocetin was prepared from gardenia by the alkaline hydrolysis method, and its reducing ability and free radical scavenging ability were tested. The in vitro anti-hypoxia vitality was studied on PC12 cells. The anti-hypoxic survival time of mice was determined in several models. The acute hypoxic injury rat model was established by simulating the hypoxic environment of 8000 m-high altitude for 24 h, and the anti-hypoxia effect of
crocetin was evaluated by intraperitoneal injection with the doses of 10, 20, and 40 mg/kg. The water contents of the brain and lung were determined, and the pathological sections in the brain, lung, heart, liver, and kidney were observed by HE staining. The levels of oxidative stress (SOD, CAT, H2O2, GSH, GSH-Px, MDA) and inflammatory factors (IL-1β, IL-6, TNF-α, VEGF) in rat brain, lung, heart, liver, and kidney tissues were detected by ELISA. The results indicated that
crocetin exhibited strong reducing ability and free radical scavenging ability and could improve the activity of PC12 cells under hypoxia. After intraperitoneal injection with crocetin, the survival time of mice was prolonged, and the pathological damage, oxidative stress, and inflammation in rats\' tissue were ameliorated. The protective activity of crocetin on vital organs in high-altitude hypoxia rats may be related to reducing oxidative stress and inhibiting inflammatory response.