背景:绷带的材料在伤口处理中起着重要作用。微生物可以定植敷料并释放毒素,在伤口中产生死亡细胞。这允许微生物结合死细胞并感染伤口。因此,需要一种能杀死绷带中细菌的敷料。为了对抗医疗保健相关的感染,医用纺织品的抗菌治疗,如纱布,制服,窗帘,床单,礼服,和面具,是必需的。此外,抗菌素耐药性是本世纪的另一个主要问题。抗菌药物的过度使用导致了耐药细菌。为了解决这两个问题,我们合成了新的有机硒化合物,可以附着在敷料的棉花上。然后我们使用了体内伤口模型,这使我们能够测量硒附着在棉花敷料上的有效性,防止细菌感染伤口.
方法:有机硒附着在棉织物上,导致织物与0.1%的硒共价连接到它。金黄色葡萄球菌(以及耐甲氧西林金黄色葡萄球菌[MRSA]),嗜麦芽窄食单胞菌,粪肠球菌,表皮葡萄球菌,选择铜绿假单胞菌进行伤口感染研究。在伤口敷料和敷料下的伤口组织中对所有细菌进行计数。在小鼠的背上造成伤口。该材料用作伤口上的绷带。将细菌注射到绷带下的伤口中。测定5天后伤口中的细菌量。使用在使用前在37°C的磷酸盐缓冲盐水中浸泡3个月的敷料材料进行类似的研究。
结果:带有硒的棉花敷料显示出完全抑制(7个对数,与对照敷料相比)不同细菌菌株,在敷料和伤口的“组织”中。使用在使用前浸泡3个月的硒棉敷料获得了类似的结果。不含硒的对照棉显示细菌完全渗入伤口和敷料。此外,根据食品和药物管理局的标准方法进行了一项研究,以显示硒杀死织物中细菌的能力,使用在洗涤剂中洗涤5次的材料。这也显示完全杀死织物中的细菌。
结论:结果表明,硒在洗涤后残留在敷料中,能够完全保护伤口免受细菌感染。在硒绷带中,5天后,绷带或伤口中未发现细菌。
BACKGROUND: The material of a bandage plays an important role in wound management. Microorganisms can colonize the dressing and release toxins, which create dead cells in the wound. This allows the microorganisms to bind the dead cells and infect the wound. Thus, a dressing is needed that kills bacteria in the bandage. To combat health care-associated infections, antimicrobial treatment of medical textiles, such as gauze, uniforms, curtains, bed sheets, gowns, and masks, is required. Besides, antimicrobial resistance is another major problem of this century. Antibacterial overuse has contributed to drug-resistant bacteria. To combat these two problems, we synthesized new organo-selenium compounds that can be attached to the cotton of the dressing. We then used an in vivo wound model, which allowed us to measure the effectiveness of selenium attached to a cotton dressing, to prevent bacteria from infecting a wound.
METHODS: Organo-selenium was attached to cotton fabric, resulting in a fabric with 0.1% selenium covalently attached to it. Staphylococcus aureus (as well as methicillin-resistant S. aureus [MRSA]), Stenotrophomonas maltophilia, Enterococcus faecalis, Staphylococcus epidermidis, and Pseudomonas aeruginosa were chosen for the wound infection study. All the bacteria were enumerated in the wound dressing and in the wound tissue under the dressing. Wounds were made on the backs of mice. The material was used as a bandage over the wound. Bacteria were injected into the wound under the bandage. The amount of bacteria in the wound after 5 days was determined. A similar study was performed using dressing material that was soaked in phosphate buffered saline at 37 °C for 3 months before use.
RESULTS: Cotton dressing with selenium attached showed complete inhibition (7 logs, as compared with control dressing) of different bacterial strains, in both the dressing and \"the tissue\" of the wound. Similar results were obtained using selenium cotton dressing that was soaked for 3 months before use. Control cotton with no selenium showed complete infiltration of bacteria into the wound and the dressing. In addition, a study was performed under Food and Drug Administration standard methods to show the ability of the selenium to kill bacteria in the fabric, using material that was washed 5 times in detergent. This also showed complete killing of bacteria in the fabric.
CONCLUSIONS: The results show that the selenium remains in the dressing after washing and is able to completely protect the wound from bacterial infection. In the selenium bandage, no bacteria were found in the bandage or the wound after 5 days.