抗生素是一类普遍存在于食品中的抗微生物药物,可有效地用于治疗疾病并促进世界范围内的动物生长。氯霉素作为一种广谱的抗革兰阳性菌和革兰阴性菌的抗生素,广泛应用于人和动物感染性疾病的有效治疗。不幸的是,氯霉素的严重副作用,比如再生障碍性贫血,肾损伤,恶心,腹泻限制了其在食品和生物医学领域的应用。开发足够灵敏的方法来检测食物中的氯霉素残留和临床诊断似乎是必不可少的需求。生物传感器已被引入作为克服这一要求的有前途的工具。作为最新类型的生物传感器之一,基于适体的生物传感器(aptasensors)是氯霉素监测的有效传感平台。在本次审查中,我们总结了可访问的aptasensor定性检测和定量测定氯霉素作为候选抗生素的最新成果。本发明的氯霉素粘附剂可以分为两个主要的光学和电化学类别。此外,其他形式的aptasensing分析,如高效液相色谱(HPLC)和微芯片电泳(MCE)已经被审查。在氯霉素传感器的制造中也突出了对利用多种纳米材料的巨大兴趣。最后,基于所研究的aptasensor的优点和缺点,提出了一些结果,为设计新型抗生素测试试剂盒提供了有希望的观点。
Antibiotics are a type of antimicrobial drug with the ubiquitous presence in foodstuff that effectively applied to treat the diseases and promote the animal growth worldwide.
Chloramphenicol as one of the antibiotics with the broad action spectrum against Gram-positive and Gram-negative bacteria is widely applied for the effective treatment of infectious diseases in humans and animals. Unfortunately, the serious side effects of
chloramphenicol, such as aplastic anemia, kidney damage, nausea, and diarrhea restrict its application in foodstuff and biomedical fields. Development of the sufficiently sensitive methods to detect
chloramphenicol residues in food and clinical diagnosis seems to be an essential demand. Biosensors have been introduced as the promising tools to overcome the requirement. As one of the newest types of the biosensors, aptamer-based biosensors (aptasensors) are the efficient sensing platforms for the
chloramphenicol monitoring. In the present review, we summarize the recent achievements of the accessible aptasensors for qualitative detection and quantitative determination of chloramphenicol as a candidate of the antibiotics. The present
chloramphenicol aptasensors can be classified in two main optical and electrochemical categories. Also, the other formats of the aptasensing assays like the high performance liquid chromatography (HPLC) and microchip electrophoresis (MCE) have been reviewed. The enormous interest in utilizing the diverse nanomaterials is also highlighted in the fabrication of the chloramphenicol aptasensors. Finally, some results are presented based on the advantages and disadvantages of the studied aptasensors to achieve a promising perspective for designing the novel antibiotics test kits.